高脂血症大鼠中富含酚类成分的水果提取物的降血脂和抗氧化作用评价及超高效液相色谱-四极杆飞行时间质谱联用技术对其化学成分的鉴定
Evaluation of Hypolipidemic and Antioxidant Effects in Phenolrich Fraction of Fruit in Hyperlipidemia Rats and Identification of Chemical Composition by Ultra-performance Liquid Chromatography Coupled with Quadropole Time-of-flight Mass Spectrometry.
作者信息
Shao Feng, Gu Lifei, Chen Huijuan, Liu Ronghua, Huang Huilian, Chen Lanying, Yang Ming
机构信息
Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, China.
Department of Complex Prescription of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, Jiangsu, China.
出版信息
Pharmacogn Mag. 2017 Oct-Dec;13(52):725-731. doi: 10.4103/pm.pm_402_16. Epub 2017 Nov 13.
BACKGROUND
Hawthorn () fruit has enjoyed a great popularity as a pleasant-tasting food associated with hypolipidemic and antioxidant effects.
OBJECTIVE
Our aim was to screen the effective fraction of hawthorn fruit in the treatment of hyperlipidemia rats. Materials and Methods: In this study, ethanol extract of hawthorn fruit (Fr.1) and four fractionated extracts (Fr.2, Fr.3, Fr.4, and Fr.5) were compared to total phenol content evaluated using Folin-Ciocalteu method, and hypolipidemic and antioxidant effects were assessed in hyperlipidemic rats.
RESULTS
Total phenol content of Fr.4 was higher than other fractions by at least 2 fold. Furthermore, this fraction possessed the strongest hypolipidemic and antioxidant effects in hyperlipidemic rats. On this basis, 15 phenolic compounds and four organic acids in Fr.4 were positively or tentatively identified using ultra-performance liquid chromatography coupled with quadropole time-of-flight mass spectrometry. In addition, 5-O-caffeoyl quinic acid butyl ester was first reported in hawthorn fruit.
CONCLUSION
Phenol-rich fraction in hawthorn fruit exhibited satisfactory hypolipidemic and antioxidant effects, and this could be exploited for further promotion of functional foods.
SUMMARY
Phenol-rich fraction in hawthorn fruit possesses most potent hypolipidemic and antioxidant effects in hyperlidemia rats. UPLC-Q-TOF-MS/MS: Ultra performance liquid chromatography coupled with quadropole time-of-flight mass spectrometry; TC: Total cholesterol; TG: Triglyceride; LDL-C: Low-density lipoprotein-cholesterol; HDL-C: High-density lipoprotein-cholesterol; GSH-Px: Glutathione peroxidase; SOD: Superoxide dismutase; MDA: Malondialdehyde; CAT: Catalase; NO: Nitric oxide; NOS: Nitric oxide synthase; ROS: Reactive oxygen species; •OOH: Superoxide anions, •OH: Hydroxyl radicals.
背景
山楂果实作为一种具有降血脂和抗氧化作用的美味食品，广受欢迎。
目的
本研究旨在筛选山楂果实治疗高脂血症大鼠的有效部位。材料与方法：本研究将山楂果实乙醇提取物（Fr.1）和4种分离提取物（Fr.2、Fr.3、Fr.4和Fr.5）进行比较，采用福林-酚法评估总酚含量，并在高脂血症大鼠中评估降血脂和抗氧化作用。
结果
Fr.4的总酚含量比其他部位至少高2倍。此外，该部位在高脂血症大鼠中具有最强的降血脂和抗氧化作用。在此基础上，采用超高效液相色谱-四极杆飞行时间质谱法对Fr.4中的15种酚类化合物和4种有机酸进行了定性或初步鉴定。此外，5-O-咖啡酰奎宁酸丁酯首次在山楂果实中报道。
结论
山楂果实中富含酚类的部位具有良好的降血脂和抗氧化作用，可用于进一步推广功能性食品。
总结
山楂果实中富含酚类的部位在高脂血症大鼠中具有最显著的降血脂和抗氧化作用。UPLC-Q-TOF-MS/MS：超高效液相色谱-四极杆飞行时间质谱；TC：总胆固醇；TG：甘油三酯；LDL-C：低密度脂蛋白胆固醇；HDL-C：高密度脂蛋白胆固醇；GSH-Px：谷胱甘肽过氧化物酶；SOD：超氧化物歧化酶；MDA：丙二醛；CAT：过氧化氢酶；NO：一氧化氮；NOS：一氧化氮合酶；ROS：活性氧；•OOH：超氧阴离子，•OH：羟基自由基。
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