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人源中脑细胞类器官移植入帕金森病小鼠模型以恢复运动功能的方案。

Protocol for transplantation of cells derived from human midbrain organoids into a Parkinson's disease mouse model to restore motor function.

机构信息

State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, IDG/McGovern, Institute for Brain Research, School of Life Sciences, Tsinghua University, Beijing 100084, China; Shandong Institute of Brain Science and Brain-inspired Research, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, China.

State Key Laboratory of Membrane Biology, Tsinghua-Peking Center for Life Sciences, IDG/McGovern, Institute for Brain Research, School of Life Sciences, Tsinghua University, Beijing 100084, China.

出版信息

STAR Protoc. 2024 Sep 20;5(3):103251. doi: 10.1016/j.xpro.2024.103251. Epub 2024 Aug 8.

DOI:10.1016/j.xpro.2024.103251
PMID:39120976
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11364011/
Abstract

Midbrain organoids provide an innovative cellular source for transplantation therapies of neurodegenerative diseases. Here, we present a protocol for midbrain organoid-derived cell transplantation into a Parkinson's disease mouse model. We describe steps for midbrain organoid generation, single-cell suspension preparation, and cell transplantation. This approach is valuable for studying the efficacy of midbrain organoids as a potential cellular source for restoring motor function. For complete details on the use and execution of this protocol, please refer to Fu et al..

摘要

中脑细胞球体为神经退行性疾病的移植治疗提供了创新性的细胞来源。在这里，我们介绍了一种将中脑细胞球体衍生的细胞移植到帕金森病小鼠模型中的方案。我们描述了中脑细胞球体的生成、单细胞悬液制备和细胞移植的步骤。这种方法对于研究中脑细胞球体作为恢复运动功能的潜在细胞来源的功效很有价值。有关使用和执行此方案的完整详细信息，请参考 Fu 等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc95/11364011/0821275676b1/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc95/11364011/4664f31836da/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc95/11364011/17c44e231f64/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc95/11364011/14bf8603118f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc95/11364011/0821275676b1/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc95/11364011/4664f31836da/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc95/11364011/17c44e231f64/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc95/11364011/14bf8603118f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc95/11364011/0821275676b1/gr3.jpg

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