Developmental and Cellular Biology, Luxembourg Centre for Systems Biomedecine (LCSB), University of Luxembourg, 6, Avenue du Swing, 4367 Belvaux, Luxembourg.
STAR Protoc. 2021 May 4;2(2):100524. doi: 10.1016/j.xpro.2021.100524. eCollection 2021 Jun 18.
The lack of advanced models recapitulating the human brain complexity is still a major obstacle in brain development and neurological disease research. Here, we describe a robust protocol to derive human midbrain organoids from neuroepithelial stem cells. These complex 3D models are characterized by the presence of functional neurons, including dopaminergic neurons and glial cells, making them particularly attractive for the study of Parkinson disease. For complete details on the use and execution of this protocol, please refer to Monzel et al. (2017).
缺乏能够重现人脑复杂性的先进模型仍然是大脑发育和神经疾病研究的主要障碍。在这里，我们描述了一种从神经上皮干细胞中获得人类中脑组织类器官的稳健方案。这些复杂的 3D 模型的特点是存在功能性神经元，包括多巴胺能神经元和神经胶质细胞，这使得它们特别适合帕金森病的研究。有关该方案使用和执行的完整详细信息，请参阅 Monzel 等人（2017 年）。
