通过旋转扩散测量得到的乳糖通透酶的大小。
The size of the lactose permease derived from rotational diffusion measurements.
作者信息
Dornmair K, Corin A F, Wright J K, Jähnig F
出版信息
EMBO J. 1985 Dec 16;4(13A):3633-8. doi: 10.1002/j.1460-2075.1985.tb04127.x.
Abstract
The lactose permease of Escherichia coli was labeled with eosinyl-maleimide, reconstituted into vesicles of dimyristoylphosphatidylcholine and subjected to time-dependent phosphorescence anisotropy measurements in order to determine the rotational diffusion coefficient. By comparison with bacteriorhodopsin, the diffusion coefficient is evaluated in terms of an effective radius of the lactose permease in the plane of the membrane. This radius amounts to 20 +/- 2 A which implies that the lactose permease is a monomer. The monomeric state is maintained in the presence of a membrane potential.
摘要
大肠杆菌的乳糖通透酶用曙红马来酰亚胺标记，重组成二肉豆蔻酰磷脂酰胆碱囊泡，并进行时间依赖性磷光各向异性测量以确定旋转扩散系数。通过与细菌视紫红质比较，根据乳糖通透酶在膜平面中的有效半径评估扩散系数。该半径为20±2埃，这意味着乳糖通透酶是单体。在存在膜电位的情况下，单体状态得以维持。
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