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1
The size of the lactose permease derived from rotational diffusion measurements.通过旋转扩散测量得到的乳糖通透酶的大小。
EMBO J. 1985 Dec 16;4(13A):3633-8. doi: 10.1002/j.1460-2075.1985.tb04127.x.
2
Evidence for phospholipid microdomain formation in liquid crystalline liposomes reconstituted with Escherichia coli lactose permease.用大肠杆菌乳糖通透酶重构的液晶脂质体中磷脂微区形成的证据。
Biophys J. 1997 Mar;72(3):1247-57. doi: 10.1016/S0006-3495(97)78771-X.
3
Fast measurement of galactoside transport by lactose permease.通过乳糖通透酶快速测量半乳糖苷转运
J Biol Chem. 1989 Jan 5;264(1):342-6.
4
From membrane to molecule with the lac permease of Escherichia coli.借助大肠杆菌的乳糖通透酶，从膜到分子
Curr Top Cell Regul. 1985;26:137-48. doi: 10.1016/b978-0-12-152826-3.50018-8.
5
Phosphatidylethanolamine is required for in vivo function of the membrane-associated lactose permease of Escherichia coli.磷脂酰乙醇胺是大肠杆菌膜相关乳糖通透酶体内功能所必需的。
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6
Internal dynamics of lactose permease.乳糖通透酶的内部动力学
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Proc Natl Acad Sci U S A. 1989 Aug;86(16):6087-91. doi: 10.1073/pnas.86.16.6087.
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[Mechanisms of efflux of a substrate accumulated by the lactose permease of Escherichia coli: theoretical and experimental study].[大肠杆菌乳糖通透酶积累的底物流出机制：理论与实验研究]
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9
Characterization of site-directed mutants in the lac permease of Escherichia coli. 2. Glutamate-325 replacements.大肠杆菌乳糖通透酶中定点突变体的特性研究。2. 谷氨酸-325的替换
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10
In vitro membrane assembly of a polytopic, transmembrane protein results in an enzymatically active conformation.一种多结构域跨膜蛋白的体外膜组装产生具有酶活性的构象。
J Cell Biol. 1989 May;108(5):1637-46. doi: 10.1083/jcb.108.5.1637.

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Lessons from lactose permease.乳糖通透酶的经验教训。
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Surface-exposed positions in the transmembrane helices of the lactose permease of Escherichia coli determined by intermolecular thiol cross-linking.通过分子间硫醇交联确定的大肠杆菌乳糖通透酶跨膜螺旋中的表面暴露位置。
Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3475-80. doi: 10.1073/pnas.052703699.
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Enhanced internal dynamics of a membrane transport protein during substrate translocation.底物转运过程中膜转运蛋白内部动力学增强。
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Rotational mobility and orientational stability of a transport protein in lipid membranes.脂质膜中转运蛋白的旋转流动性和取向稳定性。
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Topology of allosteric regulation of lactose permease.乳糖通透酶的变构调节拓扑结构
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The lipid bilayer determines helical tilt angle and function in lactose permease of Escherichia coli.脂质双分子层决定了大肠杆菌乳糖通透酶的螺旋倾斜角度和功能。
Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10167-71. doi: 10.1073/pnas.94.19.10167.
8
Properties of permease dimer, a fusion protein containing two lactose permease molecules from Escherichia coli.通透酶二聚体的特性，一种包含两个来自大肠杆菌的乳糖通透酶分子的融合蛋白。
Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5421-5. doi: 10.1073/pnas.91.12.5421.
9
The role of transmembrane domain III in the lactose permease of Escherichia coli.跨膜结构域III在大肠杆菌乳糖通透酶中的作用。
Protein Sci. 1994 Dec;3(12):2302-10. doi: 10.1002/pro.5560031215.
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Lactose permease of Escherichia coli: properties of mutants defective in substrate translocation.大肠杆菌乳糖通透酶：底物转运缺陷型突变体的特性
Proc Natl Acad Sci U S A. 1987 Aug;84(16):5535-9. doi: 10.1073/pnas.84.16.5535.

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Purification and reconstitution of functional lactose carrier from Escherichia coli.从大肠杆菌中纯化及重组功能性乳糖载体
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Rotational diffusion of biological macromolecules by time-resolved delayed luminescence (phosphorescence, fluorescence) anisotropy.通过时间分辨延迟发光（磷光、荧光）各向异性研究生物大分子的旋转扩散。
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Sequence of the lactose permease gene.乳糖通透酶基因序列。
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Morphology of proteoliposomes reconstituted with purified lac carrier protein from Escherichia coli.用从大肠杆菌中纯化的乳糖载体蛋白重构的蛋白脂质体的形态学。
J Biol Chem. 1984 Dec 25;259(24):15579-86.
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The phospholipid requirement for activity of the lactose carrier of Escherichia coli.大肠杆菌乳糖载体活性对磷脂的需求
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Purification of the lactose:H+ carrier of Escherichia coli and characterization of galactoside binding and transport.大肠杆菌乳糖：氢离子载体的纯化及半乳糖苷结合与转运特性研究
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通过旋转扩散测量得到的乳糖通透酶的大小。

The size of the lactose permease derived from rotational diffusion measurements.

作者信息

Dornmair K, Corin A F, Wright J K, Jähnig F

出版信息

EMBO J. 1985 Dec 16;4(13A):3633-8. doi: 10.1002/j.1460-2075.1985.tb04127.x.

DOI:10.1002/j.1460-2075.1985.tb04127.x
PMID:3912175
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC554707/
Abstract

The lactose permease of Escherichia coli was labeled with eosinyl-maleimide, reconstituted into vesicles of dimyristoylphosphatidylcholine and subjected to time-dependent phosphorescence anisotropy measurements in order to determine the rotational diffusion coefficient. By comparison with bacteriorhodopsin, the diffusion coefficient is evaluated in terms of an effective radius of the lactose permease in the plane of the membrane. This radius amounts to 20 +/- 2 A which implies that the lactose permease is a monomer. The monomeric state is maintained in the presence of a membrane potential.

摘要

大肠杆菌的乳糖通透酶用曙红马来酰亚胺标记，重组成二肉豆蔻酰磷脂酰胆碱囊泡，并进行时间依赖性磷光各向异性测量以确定旋转扩散系数。通过与细菌视紫红质比较，根据乳糖通透酶在膜平面中的有效半径评估扩散系数。该半径为20±2埃，这意味着乳糖通透酶是单体。在存在膜电位的情况下，单体状态得以维持。