Galanty Agnieszka, Juncewicz Paulina, Podolak Irma, Grabowska Karolina, Służały Piotr, Paśko Paweł
Department of Pharmacognosy, Jagiellonian University Medical College, Medyczna 9, 30-688 Kraków, Poland.
Department of Food Chemistry and Nutrition, Jagiellonian University Medical College, Medyczna 9, 30-688 Kraków, Poland.
Plants (Basel). 2024 Jul 23;13(15):2023. doi: 10.3390/plants13152023.
This study investigates the phytochemical composition and biological activities of hemp ( L.) leaves, flowers' methanolic extracts from the Sofia variety, and its sprouts cultivated under different light conditions (natural light, darkness, blue, and white LED light for 5, 7, and 9 days). Phytochemical analysis using HPLC identified four key polyphenolic compounds in sprouts' extracts: chlorogenic, caffeic, and gallic acids, and myricetin, with a predomination of the chlorogenic acid. In contrast, leaves and flowers' extracts contained cannflavins A and B and chlorogenic, -coumaric, and ferulic acids, with a significant presence of isochlorogenic acid. Antioxidant capacity, assessed by FRAP method, revealed higher antioxidant potential in leaves compared to flowers and sprouts, with sprouts grown under blue and white LED lights exhibiting the highest activity. Cytotoxic activity was evaluated on human colon cancer cell lines (HT29, HCT116, DLD-1) and normal colon epithelial cells (CCD 841 CoN). Results demonstrated significant and selective cytotoxicity against cancer cell lines, with leaves showing more pronounced effects than flowers, and sprouts only moderate activity. All samples revealed an anti-inflammatory effect in vitro. To conclude, sprouts, leaves, and flowers of the Sofia hemp may be considered promising products for chemoprevention in the future.
本研究调查了索非亚品种大麻（L.）的叶子、花朵的甲醇提取物及其在不同光照条件下（自然光、黑暗、蓝色和白色LED光，分别处理5、7和9天）培育的芽苗菜的植物化学成分和生物活性。使用高效液相色谱法（HPLC）进行的植物化学分析在芽苗菜提取物中鉴定出四种关键的多酚化合物：绿原酸、咖啡酸、没食子酸和杨梅素，其中绿原酸占主导地位。相比之下，叶子和花朵的提取物含有大麻黄素A和B以及绿原酸、对香豆酸和阿魏酸，异绿原酸含量显著。通过铁离子还原抗氧化能力（FRAP）法评估的抗氧化能力表明，叶子的抗氧化潜力高于花朵和芽苗菜，在蓝色和白色LED光下生长的芽苗菜表现出最高的活性。对人结肠癌细胞系（HT29、HCT116、DLD-1）和正常结肠上皮细胞（CCD 841 CoN）进行了细胞毒性活性评估。结果表明，对癌细胞系具有显著且选择性的细胞毒性，叶子的作用比花朵更明显，芽苗菜只有中等活性。所有样品在体外均显示出抗炎作用。总之，索非亚大麻的芽苗菜、叶子和花朵未来可能被视为有前景的化学预防产品。
