Stasiłowicz-Krzemień Anna, Sip Szymon, Szulc Piotr, Walkowiak Jarosław, Cielecka-Piontek Judyta
Department of Pharmacognosy and Biomaterials, Faculty of Pharmacy, Poznan University of Medical Sciences, Rokietnicka 3, 60-806 Poznan, Poland.
Department of Agronomy, Poznań University of Life Sciences, Dojazd 11, 60-632 Poznan, Poland.
Antioxidants (Basel). 2023 Oct 4;12(10):1827. doi: 10.3390/antiox12101827.
, a versatile plant with numerous varieties, holds promising potential for a wide range of biological activity. As raw materials for research, we chose leaves and inflorescences of hemp varieties such as Białobrzeskie, Henola, and Tygra, which are cultivated mainly for their fibers or seeds. The choice of extraction is a key step in obtaining the selected compositions of active compounds from plant material. Bearing in mind the lipophilic nature of cannabinoids, we performed supercritical carbon dioxide (scCO) extraction at 50 °C under 2000 (a) and 6000 PSI (b). The cannabinoid contents were determined with the use of the HPLC-DAD method. The antioxidant capabilities were assessed through a series of procedures, including the DPPH, ABTS, CUPRAC, and FRAP methods. The capacity to inhibit enzymes that play a role in the progression of neurodegenerative diseases, such as acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase was also studied. The dominant cannabinoids in the extracts were cannabidiol (CBD) and cannabidiolic acid (CBDA). The highest concentration of eight cannabinoids was detected in the Tygra inflorescences extract (b). The most notable antioxidant properties were provided by the Tygra inflorescences extract (b). Nonetheless, it was the Henola inflorescences extract (b) that demonstrated the most efficient inhibition of AChE and BChE, and tyrosinase was inhibited the most significantly by the Białobrzeskie inflorescences extract (b). Multidimensional comparative analysis enrolled all assays and revealed that the Henola inflorescences extract (b) showed the most substantial neuroprotective potential.
大麻是一种具有多种品种的多功能植物，在广泛的生物活性方面具有广阔的潜力。作为研究的原材料，我们选择了比亚沃布热斯基、赫诺拉和提格拉等大麻品种的叶子和花序，这些品种主要因其纤维或种子而种植。提取方法的选择是从植物材料中获得选定活性化合物成分的关键步骤。考虑到大麻素的亲脂性，我们在50℃、2000（a）和6000磅力/平方英寸（b）的条件下进行了超临界二氧化碳（scCO）萃取。使用高效液相色谱-二极管阵列检测法（HPLC-DAD）测定大麻素含量。通过一系列程序评估抗氧化能力，包括DPPH、ABTS、CUPRAC和FRAP方法。还研究了抑制在神经退行性疾病进展中起作用的酶的能力，如乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）和酪氨酸酶。提取物中的主要大麻素是大麻二酚（CBD）和大麻二酚酸（CBDA）。在提格拉花序提取物（b）中检测到八种大麻素的最高浓度。提格拉花序提取物（b）具有最显著的抗氧化性能。尽管如此，赫诺拉花序提取物（b）对AChE和BChE的抑制效果最为显著，而比亚沃布热斯基花序提取物（b）对酪氨酸酶的抑制作用最为明显。多维度比较分析纳入了所有测定，并表明赫诺拉花序提取物（b）具有最显著的神经保护潜力。
