酵母中GAL7基因侧翼的一个区域以及GAL4蛋白的一个结合位点作为上游激活序列。

A region flanking the GAL7 gene and a binding site for GAL4 protein as upstream activating sequences in yeast.

作者信息

Lorch Y, Kornberg R D

出版信息

J Mol Biol. 1985 Dec 20;186(4):821-4. doi: 10.1016/0022-2836(85)90400-0.

Abstract

A region of DNA 116 to 271 base-pairs upstream from the GAL7 gene of Saccharomyces cerevisiae activates transcription from a heterologous promoter and does so in either orientation, showing that the GAL7 upstream region contains an upstream activating sequence (UAS). The level of transcription obtained with two GAL7 UAS's in tandem was only 1.3 times that with one. Previous studies of the GAL1-GAL10 intergenic region were indicative of two binding sites for the GAL4 positive regulatory protein; we find that a single (synthetic) site is capable of gene activation. The level of transcription obtained with the intact GAL1-GAL10 UAS was five times that with the single site.

摘要

酿酒酵母GAL7基因上游116至271个碱基对的DNA区域可激活异源启动子的转录，且无论何种方向均可激活，这表明GAL7上游区域含有一个上游激活序列（UAS）。两个串联的GAL7 UAS所获得的转录水平仅为一个GAL7 UAS的1.3倍。先前对GAL1 - GAL10基因间区域的研究表明存在两个GAL4正调控蛋白的结合位点；我们发现一个单一的（合成）位点就能够激活基因。完整的GAL1 - GAL10 UAS所获得的转录水平是单个位点的五倍。