State Key Laboratory of Structural Analysis, Optimization and CAE Software for Industrial Equipment, School of Mechanics and Aerospace Engineering, Dalian University of Technology, Dalian, 116024, China.
Small. 2024 Nov;20(46):e2403381. doi: 10.1002/smll.202403381. Epub 2024 Aug 10.
Nanomaterials are increasingly used in biomedical imaging and cancer therapy, and how to improve the endocytosis of nanomaterials by cells is a key issue. The application of alternating current (AC) electrical stimulation to osteosarcoma cells (MG-63) here can increase the cellular endocytosis of FeO nanoparticles (diameter: 50 nm) by 52.46% via macropinocytosis. This can be ascribed to the decrease in F-actin content and the increase in intracellular Ca concentration. Transmission electron microscope, immunofluorescence staining, western blot, flow cytometry, and inductively coupled plasma emission spectrometer analyses support this interpretation. The application of electrical stimulation decreases the cell viability in magnetic hyperthermia by 47.6% and increases the signal intensity of magnetic resonance imaging by 29%. Similar enhanced endocytosis is observed for breast cancer cells (MCF-7), glioblastoma cells (U-87 MG), melanoma cells (A-375), and bladder cancer cells (TCCSUP), and also for FeO nanoparticles with the diameters of 20 and 100 nm, and ZnCoCrFeO nanoparticles with the diameter of 70 nm. It seems the electrical stimulation has the potential to improve the diagnostic and therapeutic effects of magnetic nanoparticles by promoting endocytosis.
纳米材料在生物医学成像和癌症治疗中的应用越来越广泛，如何提高纳米材料被细胞内吞的效率是一个关键问题。本研究将交流电场（AC）刺激应用于骨肉瘤细胞（MG-63），通过巨胞饮作用将 FeO 纳米颗粒（直径：50nm）的细胞内吞效率提高了 52.46%。这可以归因于 F-肌动蛋白含量的减少和细胞内 Ca 浓度的增加。透射电子显微镜、免疫荧光染色、western blot、流式细胞术和电感耦合等离子体发射光谱分析支持这一解释。电刺激应用于磁热疗可使细胞活力降低 47.6%，磁共振成像信号强度增加 29%。类似的增强内吞作用也发生在乳腺癌细胞（MCF-7）、神经胶质瘤细胞（U-87 MG）、黑色素瘤细胞（A-375）和膀胱癌细胞（TCCSUP），以及直径为 20nm 和 100nm 的 FeO 纳米颗粒，以及直径为 70nm 的 ZnCoCrFeO 纳米颗粒。似乎电刺激通过促进内吞作用有潜力提高磁性纳米颗粒的诊断和治疗效果。
