Department of Biology, The University of Iowa, Iowa City, IA, USA.
Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Methods Mol Biol. 2024;2818:195-212. doi: 10.1007/978-1-0716-3906-1_13.
The Caenorhabditis elegans germline is arranged spatiotemporally and is therefore a powerful model system for the interrogation of meiotic molecular dynamics. Coupling this property with the temporal control that the auxin-inducible degron (AID) system allows can unveil new/unappreciated roles for critical meiotic factors in specific germline regions. Here we describe a widely used approach for the introduction of degron tags to specific targets and provide a procedure for applying the AID system to C. elegans meiotic DSB repair dynamics in the germline.
秀丽隐杆线虫的生殖系在空间和时间上是有组织的，因此是探究减数分裂分子动力学的强大模型系统。将这种特性与生长素诱导的降解结构域（AID）系统允许的时间控制相结合，可以揭示关键减数分裂因子在特定生殖系区域的新的/未被充分认识的作用。在这里，我们描述了一种将降解结构域标签引入特定靶标的常用方法，并提供了一种在秀丽隐杆线虫生殖系中应用 AID 系统研究减数分裂双链断裂修复动力学的程序。
