Misugi K, Aoki I, Kikyo S, Sasaki Y, Tsunoda A, Nakajima T
Pediatr Pathol. 1985;3(2-4):217-26. doi: 10.3109/15513818509078783.
Histological sections of 36 cases of neuroblastoma and related tumors were studied with anti-S-100 protein antibody (PAP method). Schwann cells in the ganglioneuromas and ganglioneuroblastomas always strongly stained. In addition, varying numbers of spindle-shaped or elongated positively staining cells, which were probably Schwann cells and their precursor cells, were demonstrated in ganglioneuroblastoma and differentiating neuroblastoma. Undifferentiated round cell neuroblastoma showed no reaction. Immunohistochemical findings of these cases were classified into four groups (+ +, +, +/-, -) according to the number of the positive cells and compared with prognosis, histological typing, location of the tumors, stage, and age at surgery. The cases with many positive cells, group (+ +) showed excellent prognosis, and group (-) showed very poor prognosis. The results of this study indicate that S-100 protein staining provides a reliable objective method for evaluation of differentiation of the neuroblastoma cells toward Schwann cells, which appears to be an important factor to predict prognosis.
应用抗S - 100蛋白抗体（PAP法）对36例神经母细胞瘤及相关肿瘤的组织切片进行了研究。神经节神经瘤和神经节神经母细胞瘤中的施万细胞总是呈强阳性染色。此外，在神经节神经母细胞瘤和分化型神经母细胞瘤中，还发现了数量不等的梭形或细长形阳性染色细胞，这些细胞可能是施万细胞及其前体细胞。未分化的圆形细胞神经母细胞瘤无反应。根据阳性细胞数量，将这些病例的免疫组化结果分为四组（++、+、+/-、-），并与预后、组织学类型、肿瘤位置、分期及手术年龄进行比较。阳性细胞多的病例，即（++）组预后良好，而（-）组预后很差。本研究结果表明，S - 100蛋白染色为评估神经母细胞瘤细胞向施万细胞分化提供了一种可靠的客观方法，而施万细胞分化似乎是预测预后的一个重要因素。
