Jang Ji Hun, Jung Hyeon Ho, Oh Nam Su
Department of Food and Biotechnology, Korea University, Sejong, 30019 Korea.
Food Sci Biotechnol. 2024 Jun 28;33(9):2243-2254. doi: 10.1007/s10068-024-01640-x. eCollection 2024 Jul.
This study investigated the preventive potential of whey protein fermented with IM36 (FWP) against muscle atrophy induced by dexamethasone (DEX). FWP exhibited enhanced antioxidant activities compared with those of unfermented whey protein, effectively suppressing DEX-induced reactive oxygen species production. FWP was treated before the administration of 100 μM DEX on C2C12 myotubes and compared to unfermented whey (WP). DEX significantly inhibited myotube viability and muscle protein synthesis and enhanced degradation. FWP exhibited a dose-dependent attenuation of cell viability loss compared with that of WP. Additionally, FWP stimulated the formation of myotubes and muscle protein synthesis by upregulating myogenesis and insulin-like growth factor-1 expression. Furthermore, FWP significantly attenuated forkhead box protein O3a-mediated ubiquitin ligases and autophagy of lysosomes activated by DEX, inhibiting pathways that lead to muscle protein breakdown. These findings suggest that FWP enhances antioxidant activity and prevented DEX-induced muscle atrophy by regulating muscle protein homeostasis.
The online version contains supplementary material available at 10.1007/s10068-024-01640-x.
本研究调查了经IM36发酵的乳清蛋白（FWP）对由地塞米松（DEX）诱导的肌肉萎缩的预防潜力。与未发酵的乳清蛋白相比，FWP表现出增强的抗氧化活性，有效抑制DEX诱导的活性氧生成。在向C2C12肌管施用100μM DEX之前用FWP处理，并与未发酵的乳清（WP）进行比较。DEX显著抑制肌管活力和肌肉蛋白质合成并增强降解。与WP相比，FWP表现出剂量依赖性的细胞活力损失减轻。此外，FWP通过上调肌生成和胰岛素样生长因子-1表达刺激肌管形成和肌肉蛋白质合成。此外，FWP显著减弱叉头框蛋白O3a介导的泛素连接酶和由DEX激活的溶酶体自噬，抑制导致肌肉蛋白质分解的途径。这些发现表明，FWP通过调节肌肉蛋白质稳态增强抗氧化活性并预防DEX诱导的肌肉萎缩。
在线版本包含可在10.1007/s100
