Adeniyi Bolanle A, Ogunlana Mercy, Igbokwe Christopher O, Tajudeen Bamidele, Mahady Gail B
Department of Pharmaceutical Microbiology, University of Ibadan, Nigeria.
Nigerian Institute of Medical Research, Lagos, Nigeria.
Avicenna J Med Biotechnol. 2024 Jul-Sep;16(3):180-186. doi: 10.18502/ajmb.v16i3.15744.
Unlike plant phytochemicals, little has been done to explore the metabolites from phyllosphere bacterial flora, some of which enabled them to survive interspecific competition through amensalism. This study evaluated the antimicrobial activity of metabolites from Phyllospheric Bacteria (PB) isolated from (FE), against selected bacterial and fungal pathogens. Phenotypic and molecular methods were used to identify the isolated phyllo-microbiota.
The PB were aseptically isolated by sonication. Their metabolites were obtained from the fresh overnight culture of the organisms. The cell-free supernatants containing the metabolites were used for antimicrobial assays against the pathogens. The DNA of the bacterial isolates were isolated using a NIMR-BIOTECH DNA extraction kit, while their 16S rRNA was amplified with the primer: 799F 5'-AACACGGATTA GATACC-3', 1193R 5'- ACGTCATCCCCACCTTCC-3', using SolisFast* Master Mix, (Solis Biodyne-Estonia). The BLAST of the sequence was done from the NCBI Gen-bank. The PB strains identified were submitted to NCBI and accession numbers were assigned to them.
The phyllosphere of FE yielded 21 bacterial isolates: 7 Gram-positives and 14 Gram-negatives. The metabolites from these isolates showed varying degrees of bioactivity against (ATCC29213), (ATCC 25922) (ATCC 35659); , and Fifteen bioactive isolates sequenced yielded four genera, Enterobacter ( 98.44%), Bacillus ( 100%), Pontoea ( 99.72%), Staphylococcus ( 99.72%).
Bacteria from FE phyllosphere, produced metabolites antagonistic () to some human pathogens. This has great potential for possible drug discovery.
与植物的植物化学物质不同，对叶际细菌菌群的代谢产物研究较少，其中一些代谢产物能通过偏害共生在种间竞争中存活下来。本研究评估了从[具体植物名称未给出]（FE）分离出的叶际细菌（PB）代谢产物对选定细菌和真菌病原体的抗菌活性。采用表型和分子方法鉴定分离出的叶际微生物群。
通过超声处理无菌分离PB。从生物体的新鲜过夜培养物中获得其代谢产物。含有代谢产物的无细胞上清液用于对病原体进行抗菌测定。使用NIMR - BIOTECH DNA提取试剂盒分离细菌分离株的DNA，同时使用引物799F 5'-AACACGGATTAGATACC-3'、1193R 5'-ACGTCATCCCCACCTTCC-3'，利用SolisFast* Master Mix（Solis Biodyne - 爱沙尼亚）扩增其16S rRNA。序列的BLAST分析在NCBI基因库中进行。鉴定出的PB菌株提交给NCBI并为其分配登录号。
FE的叶际产生了21株细菌分离株：7株革兰氏阳性菌和14株革兰氏阴性菌。这些分离株的代谢产物对[具体细菌名称未给出]（ATCC29213）、[具体细菌名称未给出]（ATCC 25922）、[具体细菌名称未给出]（ATCC 35659）；[具体真菌名称未给出]、[具体真菌名称未给出]和[具体真菌名称未给出]表现出不同程度的生物活性。对15株有生物活性的分离株进行测序，得到四个属，即肠杆菌属（98.44%）、芽孢杆菌属（100%）、蓬托埃菌属（99.72%）、葡萄球菌属（99.72%）。
来自FE叶际的细菌产生了对某些人类病原体具有拮抗作用（[此处antagonistic后内容缺失，未明确具体所指]）的代谢产物。这在药物发现方面具有巨大潜力。
