Mazinani Zohreh, Zamani Marzieh, Sardari Soroush
Drug Design and Bioinformatics Unit, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Avicenna J Med Biotechnol. 2017 Jan-Mar;9(1):31-37.
The widespread utilization of antimicrobial compounds has caused emergence of resistant microorganisms in the world. Hence, the research to probe the products with antimicrobial features has led to finding natural habitats and discovering new pharmaceutical products.
In this study, an attempt was made to explore the niche of novel habitat to isolate pyllospheric bacteria from the above ground parts (stems and leaves) of , , , and to evaluate their antimicrobial features. The inhibitory effects of these strains on the growth of two fungi (, ), two yeasts (, ) and six bacteria (, , , , , ) were tested.
In total, 113 bacterial strains were isolated. Twenty five bacterial strains (B-1 to B-25) indicated promising antimicrobial (antibacterial and antifungal) activities against aforementioned pathogens. The identification of the bacterial strains was ascertained by morphological, physiological, biochemical tests and two strains with the strongest antimicrobial activities were further characterized based on 16s rRNA sequencing. These two strains were identified as .
Our results provide evidence that phyllospheric microorganisms are capable of producing some compounds with antimicrobial properties.
抗菌化合物的广泛使用已导致世界范围内耐药微生物的出现。因此，对具有抗菌特性的产品进行研究促使人们寻找自然栖息地并发现新的药品。
在本研究中，尝试探索新栖息地的生态位，以便从[植物名称1]、[植物名称2]、[植物名称3]和[植物名称4]的地上部分（茎和叶）分离叶际细菌，并评估它们的抗菌特性。测试了这些菌株对两种真菌（[真菌名称1]、[真菌名称2]）、两种酵母（[酵母名称1]、[酵母名称2]）和六种细菌（[细菌名称1]、[细菌名称2]、[细菌名称3]、[细菌名称4]、[细菌名称5]、[细菌名称6]）生长的抑制作用。
总共分离出113株细菌菌株。25株细菌菌株（B-1至B-25）对上述病原体显示出有前景的抗菌（抗菌和抗真菌）活性。通过形态学、生理学、生化测试确定了细菌菌株的鉴定，并且基于16s rRNA测序进一步表征了两种具有最强抗菌活性的菌株。这两种菌株被鉴定为[菌株名称]。
我们的结果提供了证据，表明叶际微生物能够产生一些具有抗菌特性的化合物。
