Rouamba Ablassé, Badini Djaouratou, Compaoré Eli, Ouédraogo Vincent, Kiendrebeogo Martin
Laboratory of Applied Biochemistry and Chemistry, Department of Biochemistry-Microbiology, UFR SVT, Université Joseph Ki-Zerbo, 03 BP 7021, Ouagadougou 03, Burkina Faso.
Ecole Normale Supérieure, 01 BP 1757, Ouagadougou 01, Burkina Faso.
Avicenna J Med Biotechnol. 2024 Jul-Sep;16(3):193-199. doi: 10.18502/ajmb.v16i3.15746.
The emergence of the multidrug-resistant bacteria strain has become a global world crisis. This study was designed to evaluate the antibiofilm and synergistic effects of leaf extracts on the activity of cefotaxime against the methicillin-resistant ().
The synergistic effect of methanol and dichloromethane extracts on the bactericidal activity of cefotaxime was determined by using the antibiotic susceptibility test on agar medium. The antibiofilm activity of the extracts was measured by using the crystal violet method. The antioxidant potential of the extracts was assessed by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Ferric Reduction Activity Potential (FRAP) methods. The main secondary metabolites groups were analyzed by using different standard analytical tests. The total phenolics and total flavonoids were quantified spectrophotometrically.
The methanol extract (final concentration of 100 ) inhibited the formation of bacterial biofilm more than salicylic acid (p<0.05). All extracts combined with cefotaxime (20 and 200 ) showed good synergistic bactericidal effect on with inhibitory diameters of up to 40 . The methanol extract showed higher total phenolics (462.20±10.90 ) and total flavonoids (26.20±0.20 ) contents than the dichloromethane extract (96.70±1.70 and 8.00±1.20 ). Moreover, the methanol extract showed a higher FRAP reducing power (353.6±4.17 ) than the dichloromethane extract (385.3±7.01 ). Qualitative phytochemical analysis showed the presence of tannins, flavonoids, terpenes and sterols in both extracts.
These data showed that leaves contain effective antibacterial phytomolecules for combating bacterial resistance.
多重耐药菌株的出现已成为全球危机。本研究旨在评估叶提取物对头孢噻肟抗耐甲氧西林（）活性的抗生物膜和协同作用。
通过琼脂培养基上的抗生素敏感性试验，测定甲醇和二氯甲烷提取物对头孢噻肟杀菌活性的协同作用。采用结晶紫法测定提取物的抗生物膜活性。采用2,2-二苯基-1-苦基肼（DPPH）法和铁还原活性电位（FRAP）法评估提取物的抗氧化潜力。通过不同的标准分析测试分析主要次生代谢物组。用分光光度法定量总酚和总黄酮。
甲醇提取物（终浓度100 ）比水杨酸更能抑制细菌生物膜的形成（p<0.05）。所有提取物与头孢噻肟（20 和200 ）联合使用时，对 均显示出良好的协同杀菌效果，抑菌直径可达40 。甲醇提取物的总酚含量（462.20±10.90 ）和总黄酮含量（26.20±0.20 ）高于二氯甲烷提取物（96.70±1.70 和8.00±1.20 ）。此外，甲醇提取物的FRAP还原能力（353.6±4.17 ）高于二氯甲烷提取物（385.3±7.01 ）。定性植物化学分析表明，两种提取物中均含有单宁、黄酮、萜类和甾醇。
这些数据表明， 叶含有有效的抗菌植物分子，可对抗细菌耐药性。
