Leaves Extracts Enhance Cefotaxime Bactericidal Effects and Quench the Biofilm Formation in Methicillin-Resistant ATCC 43300.

BACKGROUND

The emergence of the multidrug-resistant bacteria strain has become a global world crisis. This study was designed to evaluate the antibiofilm and synergistic effects of leaf extracts on the activity of cefotaxime against the methicillin-resistant ().

METHODS

The synergistic effect of methanol and dichloromethane extracts on the bactericidal activity of cefotaxime was determined by using the antibiotic susceptibility test on agar medium. The antibiofilm activity of the extracts was measured by using the crystal violet method. The antioxidant potential of the extracts was assessed by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Ferric Reduction Activity Potential (FRAP) methods. The main secondary metabolites groups were analyzed by using different standard analytical tests. The total phenolics and total flavonoids were quantified spectrophotometrically.

RESULTS

The methanol extract (final concentration of 100 ) inhibited the formation of bacterial biofilm more than salicylic acid (p<0.05). All extracts combined with cefotaxime (20 and 200 ) showed good synergistic bactericidal effect on with inhibitory diameters of up to 40 . The methanol extract showed higher total phenolics (462.20±10.90 ) and total flavonoids (26.20±0.20 ) contents than the dichloromethane extract (96.70±1.70 and 8.00±1.20 ). Moreover, the methanol extract showed a higher FRAP reducing power (353.6±4.17 ) than the dichloromethane extract (385.3±7.01 ). Qualitative phytochemical analysis showed the presence of tannins, flavonoids, terpenes and sterols in both extracts.

CONCLUSION

These data showed that leaves contain effective antibacterial phytomolecules for combating bacterial resistance.