Li Xiaowei, Cheng Jianghui, Han Haibin, Kirk William D J, O'Brien Matthew, Wang Likun, Chen Limin, Zhang Haixia, Zhang Zhijun, Ullah Farman, Desneux Nicolas, Lu Yaobin
State Key Laboratory for Managing Biotic and Chemical Threats to Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Huhhot, 010010, China.
J Chem Ecol. 2024 Dec;50(12):894-903. doi: 10.1007/s10886-024-01532-8. Epub 2024 Aug 12.
Odontothrips loti (Haliday) (Thysanoptera: Thripidae) is one of the most serious pests on alfalfa, causing direct damage by feeding and indirect damage by transmitting plant viruses. This damage causes significant loss in alfalfa production. Semiochemicals offer opportunities to develop new approaches to thrips management. In this study, behavioral responses of female and male adults of O. loti to headspace volatiles from live female and male conspecifics were tested in a Y-tube olfactometer. The results showed that both male and female adults of O. loti were attracted to the odors released by conspecific males but not those released by females. Headspace volatiles released by female and male adults were collected using headspace solid-phase microextraction (HS-SPME). The active compound in the volatiles was identified by gas chromatography-mass spectrometry (GC-MS). The analysis showed that there was one major compound, (R)-lavandulyl (R)-2-methylbutanoate. The attractive activity of the synthetic aggregation pheromone compound was tested under laboratory and field conditions. In an olfactometer, both male and female adults showed significant preference for synthetic (R)-lavandulyl (R)-2-methylbutanoate at certain doses. Lures with synthetic (R)-lavandulyl (R)-2-methylbutanoate significantly increased the trap catches of sticky white traps at doses of 40-80 µg in the field. This study confirmed the production of aggregation pheromone by O. loti male adults and identified its active compound as (R)-lavandulyl (R)-2-methylbutanoate, providing a basis for population monitoring and mass trapping of this pest.
苜蓿蓟马（Haliday）（缨翅目：蓟马科）是苜蓿上最严重的害虫之一，通过取食造成直接危害，并通过传播植物病毒造成间接危害。这种危害导致苜蓿产量大幅损失。信息化学物质为开发蓟马管理新方法提供了机会。在本研究中，在Y型嗅觉仪中测试了苜蓿蓟马雌雄成虫对来自活体同种异性个体顶空挥发物的行为反应。结果表明，苜蓿蓟马雌雄成虫均被同种雄虫释放的气味所吸引，而不被雌虫释放的气味所吸引。采用顶空固相微萃取（HS-SPME）收集雌雄成虫释放的顶空挥发物。通过气相色谱-质谱联用（GC-MS）鉴定挥发物中的活性化合物。分析表明，有一种主要化合物，即（R）-薰衣草基（R）-2-甲基丁酸酯。在实验室和田间条件下测试了合成聚集信息素化合物的引诱活性。在嗅觉仪中，雌雄成虫在一定剂量下对合成的（R）-薰衣草基（R）-2-甲基丁酸酯均表现出显著偏好。在田间，含有合成（R）-薰衣草基（R）-2-甲基丁酸酯的诱芯在40-80微克剂量下显著增加了白色粘虫板的诱捕量。本研究证实了苜蓿蓟马雄虫产生聚集信息素，并鉴定其活性化合物为（R）-薰衣草基（R）-2-甲基丁酸酯，为该害虫的种群监测和大量诱捕提供了依据。
