Department of Pediatrics, Section of Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Methods Mol Biol. 2024;2831:1-9. doi: 10.1007/978-1-0716-3969-6_1.
Primary neuronal cultures allow for in vitro analysis of early developmental processes such as axon pathfinding and growth dynamics. When coupled with methods to visualize and measure microtubule dynamics, this methodology enables an inside look at how the cytoskeleton changes in response to extracellular signaling cues. Here, we describe the culturing conditions and tools required to extract primary cortical neurons from postnatal mouse brains and visualize cytoskeletal components.
原代神经元培养允许对轴突导向和生长动力学等早期发育过程进行体外分析。当与可视化和测量微管动力学的方法结合使用时，这种方法能够深入了解细胞骨架如何响应细胞外信号线索发生变化。在这里，我们描述了从新生小鼠大脑中提取原代皮质神经元并可视化细胞骨架成分所需的培养条件和工具。
