Indian Institute of Science Education and Research (IISER) Pune, Pune, India.
INSERM UMR-S 1270/ Sorbonne University/ Institut du Fer à Moulin, Paris, France.
Methods Mol Biol. 2024;2831:251-264. doi: 10.1007/978-1-0716-3969-6_17.
Growth cone-dependent outgrowth of neuronal processes is essential for the development, plasticity, and regenerative capacity of the nervous system. This process involves the attachment of the growth cone to the substrate and the cyclical engagement/disengagement of the molecular clutch at the sites of adhesive contact. In this chapter, we describe protocols for traction force microscopy, measurement of F-actin retrograde flow velocities, and the assessment of adhesive point contacts by immunofluorescence. These complementary techniques collectively facilitate investigations into the regulation of the molecular clutch in neuronal growth cones.
神经元突起的生长锥依赖性生长对于神经系统的发育、可塑性和再生能力至关重要。这个过程涉及到生长锥与基底的附着，以及在黏附接触部位分子离合器的周期性结合/分离。在本章中，我们描述了牵引力显微镜、测量 F-肌动蛋白逆行流动速度以及免疫荧光法评估黏附点接触的实验方案。这些互补技术共同促进了对神经元生长锥中分子离合器调控的研究。
