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采用紫外可见分光光度法和 UPLC-TQ-MS 技术分析不同品种玫瑰果实的抗氧化性能和化合物。

Analysis of Antioxidant Properties and Compounds in Different Rosa Taxa Fruits Using UV-Vis and UPLC-TQ-MS Techniques.

机构信息

Liaoning Provincial Tumor Hospital, Affiliated Tumor Hospital of Dalian University of Technology, Shenyang, Liaoning province, China.

Department of Medicine, Dalian University of Technology, Dalian, Liaoning province, China.

出版信息

Chem Biodivers. 2024 Nov;21(11):e202401029. doi: 10.1002/cbdv.202401029. Epub 2024 Oct 8.

Abstract

This comprehensive analysis of the fruits of Rosa spp. (FR) evaluates their chemical components and antioxidant activity. The study quantified total flavonoids and polyphenols using aluminum trichloride colorimetric assay and Folin-Ciocalteu methods, with the fruit of Rosa. laxa Rtez. var. mollis Yü et Ku. sample exhibiting the highest concentrations of 59.21 mg/g and 81.13 mg/g, respectively. Ultra-High-Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry (UPLC-TQ-MS) assessed seven primary components, with notable levels of euscaphic acid, ursolic acid, and gallic acid. Antioxidant activities were tested using DPPH and ABTS methods, showing strong activities in samples the fruits of Rosa. persica Mickx ex Juss. and Rosa. laxa Rtez. var. kaschgarica (Rupr.) Y. L. Han. Chemometric analyses, including similarity, cluster, principal component, and grey relational analyses, were used to explore relationships between FR varieties and their antioxidant properties. The study provides a vital basis for future FR quality assessments.

摘要

本研究对蔷薇属(FR)果实进行了全面分析,评估了其化学组成和抗氧化活性。采用三氯化铝比色法和 Folin-Ciocalteu 法测定总黄酮和多酚的含量,其中软条七蔷薇果实的浓度最高,分别为 59.21mg/g 和 81.13mg/g。采用超高效液相色谱-三重四极杆质谱联用(UPLC-TQ-MS)测定了 7 种主要成分,其中 euscaphic 酸、熊果酸和没食子酸的含量较高。采用 DPPH 和 ABTS 法测定抗氧化活性,显示出蔷薇属果实和软条七蔷薇果实的抗氧化活性较强。化学计量学分析,包括相似性、聚类、主成分和灰色关联分析,用于探讨 FR 品种与其抗氧化特性之间的关系。该研究为今后 FR 的质量评估提供了重要依据。

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