VPS72在结肠癌中的表达分析及相关生物学行为

Expression Analysis of VPS72 and Associated Biological Behaviors in Colon Cancer.

作者信息

Cao Jun, Zhang Hao, Xie Xin, Wang Wei

机构信息

Department of Oncology, The Third Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi, 710061, People's Republic of China.

Department of Surgical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, People's Republic of China.

出版信息

Int J Gen Med. 2024 Aug 8;17:3433-3442. doi: 10.2147/IJGM.S465064. eCollection 2024.

DOI:10.2147/IJGM.S465064

PMID:39135633

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11318599/

Abstract

BACKGROUND

VPS72 is highly expressed in hepatocellular carcinoma and prostate cancer, participating in various cellular processes such as gene transcription, replication, DNA repair, maintenance of genome integrity, and cancer progression. However, its role in colorectal cancer remains unknown.

METHODS

Bioinformatic methods were used to analyze gene expression, correlation and patient survival. Western blotting, colony formation assays and animal experiments were used to evaluate the function of VPS72 in colon cancer in vivo and in vitro.

RESULTS

VPS72 was highly expressed in colon cancer tissues and correlated with poor overall survival (<0.05) and relapse free survival (<0.01). Furthermore, patients with III/IV clinical stage (<0.001), N1 nodal metastasis (<0.001) or N2 nodal metastasis (<0.05) status had poor overall survival. Further analysis showed that VPS72 is correlated with proliferation and EMT biomarkers. Western blotting, colony formation assays and animal experiments showed that VPS72 overexpression promoted colon cancer proliferation and EMT progress.

CONCLUSION

Our study found that VPS72 was correlated with poor overall survival in colon cancer patients, and high expressed level of VPS72 promoted colon cancer progression, indicating its role as a potential prognosis biomarker.

摘要

背景

VPS72在肝细胞癌和前列腺癌中高表达，参与基因转录、复制、DNA修复、基因组完整性维持及癌症进展等多种细胞过程。然而，其在结直肠癌中的作用尚不清楚。

方法

采用生物信息学方法分析基因表达、相关性及患者生存情况。运用蛋白质免疫印迹法、集落形成试验及动物实验评估VPS72在体内外结肠癌中的功能。

结果

VPS72在结肠癌组织中高表达，与总生存期较差（<0.05）及无复发生存期较差（<0.01）相关。此外，临床分期为III/IV期（<0.001）、N1淋巴结转移（<0.001）或N2淋巴结转移（<0.05）状态的患者总生存期较差。进一步分析表明，VPS72与增殖及上皮-间质转化生物标志物相关。蛋白质免疫印迹法、集落形成试验及动物实验表明，VPS72过表达促进结肠癌增殖及上皮-间质转化进程。