Yu Jie, Li Weicheng, Xu Ruibo, Liu Xiaoye, Gao Guangqi, Kwok Lai-Yu, Chen Yongfu, Sun Zhihong, Liu Wenjun, Zhang Heping
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education Inner Mongolia Agricultural University Hohhot China.
Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs Inner Mongolia Agricultural University Hohhot China.
Imeta. 2024 Jul 9;3(4):e224. doi: 10.1002/imt2.224. eCollection 2024 Aug.
Breast milk naturally contains lactic acid bacteria, but their precise origin remains a subject of debate. In this study, we utilized a rat mastitis animal model to investigate the potential of a breast milk-derived probiotic strain, Probio-M9, in alleviating mastitis and enhancing the efficacy of antibiotic treatment. Through histopathological analysis of mammary tissue, we observed that Probio-M9 effectively relieved mastitis, mitigated inflammation, and improved the response to antibiotic treatment. Metagenomic analysis further revealed that Probio-M9 enhanced interactions among gut microbes, accompanied by an increase in the relative abundance of and the regulation of specific genes and carbohydrate-active enzymes, subsequently impacting host immunity. Additionally, an intriguing finding was the translocation of live Probio-M9 from the gut to the mammary tissue only during bacterial mastitis and lactation, likely facilitated through lymphatic circulation. These findings advance our understanding of the intricate gut-mammary axis and provide valuable insights into the potential health benefits of probiotic interventions.
母乳中天然含有乳酸菌，但其确切来源仍是一个有争议的话题。在本研究中，我们利用大鼠乳腺炎动物模型来研究一种源自母乳的益生菌菌株Probio-M9在缓解乳腺炎和提高抗生素治疗效果方面的潜力。通过对乳腺组织的组织病理学分析，我们观察到Probio-M9有效地缓解了乳腺炎，减轻了炎症，并改善了对抗生素治疗的反应。宏基因组分析进一步表明，Probio-M9增强了肠道微生物之间的相互作用，伴随着 相对丰度的增加以及特定基因和碳水化合物活性酶的调节，随后影响宿主免疫力。此外，一个有趣的发现是，只有在细菌性乳腺炎和哺乳期期间，活的Probio-M9才会从肠道转移到乳腺组织，可能是通过淋巴循环促进的。这些发现增进了我们对复杂的肠道-乳腺轴的理解，并为益生菌干预的潜在健康益处提供了有价值的见解。
