Tank B, Marquet R L, Vennemans E, Westbroek D L
Eur J Cancer Clin Oncol. 1985 Dec;21(12):1539-46. doi: 10.1016/0277-5379(85)90249-4.
The tube leukocyte adherence inhibition (LAI) assay was used to follow the LAI response in inbred BN rats with subcutaneously (s.c.) transplanted syngeneic liposarcoma (LS175) tumor and in inbred WAG rats with s.c. transplanted syngeneic colon (CC531) and skin (1618) tumors. Initially, the tube LAI assay was used to follow the LAI reactivity of peripheral blood leukocytes (PBL) from BN rats bearing tumor LS175. Sporadic LAI reactivity was observed in the PBL of these rats when incubated with a specific crude tumor extract for either 2 or 20 hr. No definite conclusion regarding the lack of significant tumor-specific LAI reactivity could be drawn since LS175 is a non-immunogenic tumor. Therefore additional LAI studies were performed with the weakly immunogenic tumor CC531 and the highly immunogenic tumor 1618 in WAG rats. Surprisingly, only sporadic LAI reactivity was observed in the PBL of rats bearing either the CC531 or the 1618 tumors using both the 2 and 20 hr LAI assays. This absence of tumor-specific LAI reactivity was particularly remarkable in the highly immunogenic 1618 tumor-bearing rats, since it would suggest that the antigen(s) responsible for its high immunogenicity could not evoke any consistent LAI reactivity. Since oxidative metabolites of arachidonic acid have been shown to be the final mediators of chemoattractant-induced LAI, the adherence inhibition effect of leukotriene B4 (LTB4) on the PBL of BN and WAG rats was also investigated in order to exclude the possibility that the lack of consistent LAI reactivity was due to a lack of responsive cell population in the PBL. PBL of both BN and WAG rats showed a significant increase in the adherence inhibition in the presence of LTB4, suggesting that the sporadic LAI reactivity was not due to a lack of responsive cell population.
采用试管白细胞黏附抑制（LAI）试验，对皮下（s.c.）移植同基因脂肪肉瘤（LS175）肿瘤的近交系BN大鼠以及皮下移植同基因结肠（CC531）和皮肤（1618）肿瘤的近交系WAG大鼠的LAI反应进行跟踪研究。最初，使用试管LAI试验跟踪荷瘤LS175的BN大鼠外周血白细胞（PBL）的LAI反应性。当将这些大鼠的PBL与特定的粗肿瘤提取物孵育2小时或20小时时，在其PBL中观察到散在的LAI反应性。由于LS175是一种非免疫原性肿瘤，因此无法就缺乏显著的肿瘤特异性LAI反应性得出明确结论。因此，对WAG大鼠中弱免疫原性肿瘤CC531和强免疫原性肿瘤1,618进行了额外的LAI研究。令人惊讶的是，使用2小时和20小时的LAI试验，在荷瘤CC531或1,618的大鼠的PBL中均仅观察到散在的LAI反应性。在强免疫原性的荷瘤1,618大鼠中，这种缺乏肿瘤特异性LAI反应性的情况尤为显著，因为这表明负责其高免疫原性的抗原无法引发任何一致的LAI反应性。由于花生四烯酸的氧化代谢产物已被证明是趋化因子诱导的LAI的最终介质，因此还研究了白三烯B4（LTB4）对BN和WAG大鼠PBL的黏附抑制作用，以排除缺乏一致的LAI反应性是由于PBL中缺乏反应性细胞群体的可能性。BN和WAG大鼠PBL在LTB4存在下的黏附抑制均显著增加，表明散在的LAI反应性并非由于缺乏反应性细胞群体所致。
