Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru.
Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru.
Am J Trop Med Hyg. 2024 Aug 13;111(4):823-825. doi: 10.4269/ajtmh.24-0171. Print 2024 Oct 2.
Neurocysticercosis is a parasitic disease of major public health importance. Definitive diagnosis requires neuroimaging, which is typically unavailable in rural impoverished regions of endemicity. Screening immunoassays can support diagnosis in this setting by identifying individuals most likely to have severe forms of disease for referral to imaging. Urine sampling is convenient, painless, and generally well accepted. We developed a rapid point-of-care (POC) assay to detect urinary antigens and assessed concordance with a standard antigen ELISA (Ag-ELISA), both using monoclonal antibodies TsW8/TsW5. From 28,145 stored community samples with Ag-ELISA results, we selected 843 for comparison, 281 each from nonreactive (ratio <1), reactive-below-cutoff (ratio 1:3), and positive (ratio ≥3) samples. Overall agreement was 73.6%, with strong agreement observed in the nonreactive (280/281, 99.6%) and positive (255/281, 90.8%) groups. This affordable noninvasive POC test can be applied to identify individuals in the community most at risk of developing severe disease.
脑囊虫病是一种具有重要公共卫生意义的寄生虫病。明确诊断需要神经影像学检查，但在流行地区的农村贫困地区通常无法获得。筛选免疫检测可以通过识别最有可能患有严重疾病的个体，从而支持在这种情况下进行诊断，以便将其转诊进行影像学检查。尿液采样方便、无痛，且通常被广泛接受。我们开发了一种快速即时检测（POC）检测方法来检测尿液抗原，并评估了其与使用单克隆抗体 TsW8/TsW5 的标准抗原酶联免疫吸附试验（Ag-ELISA）的一致性。从具有 Ag-ELISA 结果的 28,145 份储存的社区样本中，我们选择了 843 份进行比较，每份样本分别来自无反应（比值 <1）、反应低于临界值（比值 1:3）和阳性（比值≥3）样本。总体一致性为 73.6%，在无反应（280/281，99.6%）和阳性（255/281，90.8%）组中观察到了很强的一致性。这种经济实惠的非侵入性 POC 测试可用于识别社区中最有可能患严重疾病的个体。
