Škach Jiřĺ, Šlamborová Irena, Hromádka Peter, Exnar Petr, Gürlich Robert
Department of Surgery, Regional Hospital Liberec a.s., Liberec, Czech Republic.
Department of Chemistry, Technical University of Liberec, Liberec, Czech Republic.
Ann Surg Treat Res. 2024 Aug;107(2):108-119. doi: 10.4174/astr.2024.107.2.108. Epub 2024 Jul 30.
The aim of this study was to evaluate the antibacterial efficacy of surface-treated hernia implants modified by a hybrid nanolayer with incorporated Ag, Cu, and Zn cations using the sol-gel method.
The materials (polypropylene, polyester, and polyvinylidene difluoride) were activated by vacuum plasma treatment or UV C radiation, then modified and tested for bacterial strains of (gram-negative) and (gram-positive). The AATCC 100 (2019) method for quantitative and the ISO 20645 agar plate propagation method for qualitative evaluation of microbiological efficacy were used. The gradual release of incorporated ions was monitored over time in simulated body fluids (blood plasma, peritoneal fluid) and physiological saline using an inductively coupled plasma mass spectrometer. The thickness and the homogeneity of the layers were measured for individual random samples with scanning electron microscope analysis (SEMA) and evaluated with an elemental analysis.
Qualitative and quantitative microbiological tests clearly show the great suitability of vacuum plasma and UV C with sol AD30 (dilution 1:1) surface treatment of the implants. The absolute concentration of Ag, Cu, and Zn cations in leachates was very low. SEMA showed a high degree of homogeneity of the layer and only very rare nanocracks by all tested materials appear after mechanical stress.
This study confirms that surface treatment of meshes using the sol-gel method significantly increases the antibacterial properties. The nanolayers are sufficiently mechanically resistant and stable and pose no threat to health.
本研究旨在评估采用溶胶 - 凝胶法制备的、含有银、铜和锌阳离子的混合纳米层修饰的表面处理疝修补植入物的抗菌效果。
材料（聚丙烯、聚酯和聚偏二氟乙烯）通过真空等离子体处理或紫外线C辐射进行活化，然后进行修饰，并针对革兰氏阴性菌和革兰氏阳性菌菌株进行测试。采用美国纺织化学家和染色家协会100（2019）定量方法以及ISO 20645琼脂平板繁殖法定性评估微生物学效果。使用电感耦合等离子体质谱仪监测模拟体液（血浆、腹膜液）和生理盐水中掺入离子随时间的逐渐释放情况。通过扫描电子显微镜分析（SEMA）测量各个随机样品层的厚度和均匀性，并进行元素分析评估。
定性和定量微生物学测试清楚地表明，真空等离子体和紫外线C结合溶胶AD30（稀释比例1:1）对植入物进行表面处理具有很大的适用性。渗滤液中银、铜和锌阳离子的绝对浓度非常低。SEMA显示层具有高度均匀性，并且所有测试材料在机械应力后仅出现非常罕见的纳米裂纹。
本研究证实，采用溶胶 - 凝胶法对补片进行表面处理可显著提高抗菌性能。纳米层具有足够的机械抗性和稳定性，对健康无威胁。
