Targeted elimination of alleviates periodontitis.

BACKGROUND

, a pathobiont in periodontal disease, contributes to alveolar bone destruction. We assessed the efficacy of a new targeted antimicrobial, FP-100, in eradicating from the oral microbial community and and evaluated its effectiveness in reducing bone loss in a mouse periodontitis model.

METHODS

A multispecies bacterial community was cultured and treated with two concentrations of FP-100 over two days. Microbial profiles were examined at 24-h intervals using 16S rRNA sequencing. A ligature-induced periodontitis mouse model was employed to test FP-100 in vivo.

RESULTS

FP-100 significantly reduced . within the in vitro community ( < 0.05) without altering microbial diversity at a 2 μM concentration. In mice, cultivable was undetectable in FP-100-treated ligatures but persistent in controls. Beta diversity plots showed distinct microbial structures between treated and control mice. Alveolar bone loss was significantly reduced in the FP-100 group ( = 0.018), with concurrent decreases in gingival IL-1β and TNF-α expression ( = 0.052 and 0.018, respectively).

CONCLUSION

FP-100 effectively eliminates from oral microbiota and significantly reduces bone loss in a mouse periodontitis model, demonstrating its potential as a targeted therapeutic agent for periodontal disease.