Department of Physiology and Membrane Biology, University of California Davis School of Medicine, Davis, Davis, CA, USA.
Bioinformatics Core, Genome Center, University of California Davis, Davis, CA, USA.
Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4.
The Neuroscience Monoclonal Antibody Sequencing Initiative (NeuroMabSeq) is a concerted effort to determine and make publicly available hybridoma-derived sequences of monoclonal antibodies (mAbs) valuable to neuroscience research. Over 30 years of research and development efforts including those at the UC Davis/NIH NeuroMab Facility have resulted in the generation of a large collection of mouse mAbs validated for neuroscience research. To enhance dissemination and increase the utility of this valuable resource, we applied a high-throughput DNA sequencing approach to determine immunoglobulin heavy and light chain variable domain sequences from source hybridoma cells. The resultant set of sequences was made publicly available as a searchable DNA sequence database (neuromabseq.ucdavis.edu) for sharing, analysis and use in downstream applications. We enhanced the utility, transparency, and reproducibility of the existing mAb collection by using these sequences to develop recombinant mAbs. This enabled their subsequent engineering into alternate forms with distinct utility, including alternate modes of detection in multiplexed labeling, and as miniaturized single chain variable fragments or scFvs. The NeuroMabSeq website and database and the corresponding recombinant antibody collection together serve as a public DNA sequence repository of mouse mAb heavy and light chain variable domain sequences and as an open resource for enhancing dissemination and utility of this valuable collection of validated mAbs.
神经单克隆抗体测序计划（NeuroMabSeq）是一项协同努力，旨在确定并公开可用于神经科学研究的杂交瘤衍生的单克隆抗体（mAbs）序列。超过 30 年的研究和开发工作，包括加州大学戴维斯分校/ NIH 神经 Mab 设施的工作，产生了大量经过验证可用于神经科学研究的小鼠 mAbs。为了增强传播效果并增加此有价值资源的实用性，我们应用高通量 DNA 测序方法从原始杂交瘤细胞中确定免疫球蛋白重链和轻链可变区序列。将得到的序列集作为可搜索的 DNA 序列数据库（neuromabseq.ucdavis.edu）公开，用于共享、分析和下游应用。我们通过使用这些序列开发重组 mAbs 来增强现有 mAb 集合的实用性、透明度和可重复性。这使得它们能够随后被工程化为具有不同用途的替代形式，包括在多重标记中进行不同的检测方式，以及作为微型化的单链可变片段或 scFvs。NeuroMabSeq 网站和数据库以及相应的重组抗体集合共同充当小鼠 mAb 重链和轻链可变区序列的公共 DNA 序列存储库，并作为增强此经过验证的 mAb 宝贵集合的传播和实用性的开放资源。
