Cheng Mengxin, Cao Ying, Chen Lifen, Lin Yue, Luo Fang, Guo Longhua, Qiu Bin, Lin Zhenyu
Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fujian 350116, China.
College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, Zhejiang 314001, China.
ACS Sens. 2025 Jun 27;10(6):4580-4588. doi: 10.1021/acssensors.5c00871. Epub 2025 Jun 3.
Histamine is a kind of biogenic amine released in spoiled food, which can serve as a key indicator for assessing the freshness of seafood. It is important to develop some efficient methods for histamine detection. In this work, a dual-emission europium-metal-organic framework (Eu-MOF) was designed and applied to design a ratiometric fluorescence sensing strategy for histamine detection. The MOF was synthesized in one step using two ligands: 5-boronoisophthalic acid (5-BOP) and 2-hydroxyterephthalic acid (BDC-OH). Eu was sensitized by 5-BOP through the antenna effect and exhibited red emission at 618 nm, while the BDC-OH exhibited bright blue emission at 434 nm. The MOF displayed dual-emission responses to histamine, characterized by an increase in 434 nm and a concomitant decrease at 618 nm, so the ratio of / decreased with a pronounced fluorescence color shift from purple to blue. / exhibited a linear range with histamine from 0 to 200 mg/L with a detection limit of 0.17 mg/L (LOD = 3σ/K). The synthesized Eu-BOP/BDC-OH was integrated with carrageenan to fabricate a hydrogel composite film for portable detection of histamine, and the changing of color can be utilized for semiquantitative freshness detection. The proposed method provided a reliable and efficient portable strategy for histamine detection in seafood.
组胺是一种在变质食品中释放的生物胺，可作为评估海鲜新鲜度的关键指标。开发一些高效的组胺检测方法很重要。在这项工作中，设计了一种双发射铕金属有机框架（Eu-MOF）并将其应用于设计用于组胺检测的比率荧光传感策略。该MOF使用两种配体一步合成：5-硼酸间苯二甲酸（5-BOP）和2-羟基对苯二甲酸（BDC-OH）。Eu通过天线效应被5-BOP敏化并在618nm处呈现红色发射，而BDC-OH在434nm处呈现亮蓝色发射。该MOF对组胺表现出双发射响应，其特征是434nm处增加而618nm处同时降低，因此/的比率随着荧光颜色从紫色到蓝色的明显变化而降低。/与组胺的线性范围为0至200mg/L，检测限为0.17mg/L（LOD = 3σ/K）。将合成的Eu-BOP/BDC-OH与卡拉胶整合以制备用于组胺便携式检测的水凝胶复合膜，颜色变化可用于半定量新鲜度检测。所提出的方法为海鲜中组胺的检测提供了一种可靠且高效的便携式策略。
