Wu Wenming, Liu Jian, Ruan Hanli, Jin An
Department of Pharmacy, Jiangxi provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College, Nanchang 330006, China.
School of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua 418000, China; School of Pharmaceutical Science, University of South China, Hengyang 421001, Hunan Province, China.
Fitoterapia. 2024 Oct;178:106184. doi: 10.1016/j.fitote.2024.106184. Epub 2024 Aug 12.
The phytochemical investigations on the fruits of Kadsura coccinea led to the isolation of six undescribed dibenzocyclooctadiene lignans named kadcolignans B-G, together with eleven previously described analogues. The structures of these compounds were established by spectroscopic methods including NMR, HRESIMS, and CD experiments. All isolated compounds were evaluated for their hepatoprotective activity by measuring the levels of triglyceride (TG), total cholesterol (TC), and reactive oxygen species (ROS) in FFA-induced HepG2 cells. As a result, compounds 4, 5, 9, 13, and 15 showed potent inhibitory effects on hepatocyte lipid accumulation at a concentration of 100 μM. Our research not only broadens the understanding on the chemical composition of K. coccinea but also provides experimental and theoretical evidences supporting the fruit's active ingredients in alleviating nonalcoholic fatty liver disease (NAFLD).
对黑老虎果实进行的植物化学研究，导致分离出六种未描述的二苯并环辛二烯木脂素，命名为黑老虎木脂素B - G，以及十一种先前已描述的类似物。这些化合物的结构通过包括核磁共振（NMR）、高分辨电喷雾电离质谱（HRESIMS）和圆二色（CD）实验在内的光谱方法得以确定。通过测量游离脂肪酸（FFA）诱导的HepG2细胞中甘油三酯（TG）、总胆固醇（TC）和活性氧（ROS）的水平，对所有分离出的化合物进行了肝保护活性评估。结果，化合物4、5、9、13和15在浓度为100μM时对肝细胞脂质积累显示出强效抑制作用。我们的研究不仅拓宽了对黑老虎化学成分的认识，还提供了实验和理论证据，支持该果实的活性成分在缓解非酒精性脂肪性肝病（NAFLD）方面的作用。
