TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Materia Medica Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, PR China.
Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Key Laboratory of Colleges and Universities in Hunan Province for Cytobiology and Molecular Biotechnology, Hunan University of Chinese Medicine, Changsha, Hunan, 410208, PR China.
Phytochemistry. 2021 Apr;184:112678. doi: 10.1016/j.phytochem.2021.112678. Epub 2021 Feb 4.
Phytochemical investigations on the fresh fruits of Kadsura coccinea (Lem.) A. C. Sm. have led to the isolation of fourteen undescribed 2,2'-cyclolignans named heilaohuguosus A-N, four undescribed aryltetrahydronaphthalene lignans, heilaohuguosus O-R and one tetrahydrofuran lignan, heilaohuguosu S, with twenty-seven previously described lignan analogues. Their structures and absolute configurations of heilaohuguosus A-S were established by spectroscopic methods including 1D and 2D-NMR techniques and CD experiments. All isolated compounds were evaluated for their hepatoprotective activity against APAP-induced toxicity in HepG-2 cells, four 2,2'-cyclolignans, heilaohuguosus A and L, tiegusanin I and kadsuphilol I showed good hepatoprotective activities against APAP toxicity in HepG-2 cells with cell survival rates of 53.5 ± 1.7%, 55.2 ± 1.2%, 52.5 ± 2.4%, and 54.0 ± 2.2% (positive control bicyclol, 52.1 ± 1.3%) at 10 μM, respectively.
从新鲜的三叶木通（Kadsura coccinea（Lem.）A. C. Sm.）果实中进行植物化学研究，导致分离出 14 种未描述的 2,2'-环木脂素，命名为黑老胡骨素 A-N，4 种未描述的芳基四氢萘木脂素，黑老胡骨素 O-R 和 1 种四氢呋喃木脂素，黑老胡骨素 S，以及 27 种以前描述的木脂素类似物。通过包括 1D 和 2D-NMR 技术和 CD 实验在内的光谱方法确定了黑老胡骨素 A-S 的结构和绝对构型。所有分离出的化合物都针对它们在 HepG-2 细胞中对 APAP 诱导的毒性的肝保护活性进行了评估，其中 4 种 2,2'-环木脂素，黑老胡骨素 A 和 L、替古苏林 I 和卡杜菲洛 I 对 HepG-2 细胞中的 APAP 毒性表现出良好的肝保护活性，细胞存活率分别为 53.5±1.7%、55.2±1.2%、52.5±2.4%和 54.0±2.2%（阳性对照双环醇为 52.1±1.3%），在 10μM 时。
