Scholler Jules, Groux Kassandra, Goureau Olivier, Sahel José-Alain, Fink Mathias, Reichman Sacha, Boccara Claude, Grieve Kate
Institut Langevin, ESPCI Paris, PSL University, CNRS, 10 rue Vauquelin, Paris, France.
Institut de la Vision, Sorbonne Université, INSERM, CNRS, F-75012 Paris, France.
Light Sci Appl. 2020 Aug 17;9:140. doi: 10.1038/s41377-020-00375-8. eCollection 2020.
Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue but lacks functional information. We present dynamic full-field optical coherence tomography as a technique to noninvasively image living human induced pluripotent stem cell-derived retinal organoids. Coloured images with an endogenous contrast linked to organelle motility are generated, with submicrometre spatial resolution and millisecond temporal resolution, creating a way to identify specific cell types in living tissue via their function.
光学相干断层扫描技术为成像活体组织的复杂结构提供了惊人的机会，但缺乏功能信息。我们提出了动态全场光学相干断层扫描技术，作为一种对源自人诱导多能干细胞的视网膜类器官进行无创成像的技术。生成了与细胞器运动相关的具有内源性对比度的彩色图像，具有亚微米级的空间分辨率和毫秒级的时间分辨率，创造了一种通过功能识别活体组织中特定细胞类型的方法。
