Li Wanglu, Yao Zhihao, Ma Tongtong, Ye Zihong, He Kaiyu, Wang Liu, Wang Hongmei, Fu Yingchun, Xu Xiahong
College of Life Science, China Jiliang University, Hangzhou 310018, China; Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; College of Biosystems Engineering and Food Science, Key Laboratory of Intelligent Equipment and Robotics for Agriculture of Zhejiang Province, Zhejiang University, Hangzhou 310058, China.
Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; Lab of Brewing Microbiology and Applied Enzymology, The Key Laboratory of Industrial Biotechnology, Ministry of Education, State Key Laboratory of Food Science and Technology, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China.
Adv Colloid Interface Sci. 2024 Oct;332:103276. doi: 10.1016/j.cis.2024.103276. Epub 2024 Aug 12.
Acoustofluidic technologies that integrate acoustic waves and microfluidic chips have been widely used in bioparticle manipulation. As a representative technology, acoustic tweezers have attracted significant attention due to their simple manufacturing, contact-free operation, and low energy consumption. Recently, acoustic tweezers have enabled the efficient and smart manipulation of biotargets with sizes covering millimeters (such as zebrafish) and nanometers (such as DNA). In addition to acoustic tweezers, other related acoustofluidic chips including acoustic separating, mixing, enriching, and transporting chips, have also emerged to be powerful platforms to manipulate micro/nano bioparticles (cells in blood, extracellular vesicles, liposomes, and so on). Accordingly, some interesting applications were also developed, such as smart sensing. In this review, we firstly introduce the principles of acoustic tweezers and various related technologies. Second, we compare and summarize recent applications of acoustofluidics in bioparticle manipulation and sensing. Finally, we outlook the future development direction from the perspectives such as device design and interdisciplinary.
将声波与微流控芯片相结合的声流控技术已广泛应用于生物粒子操控领域。作为一项代表性技术，声镊因其制造简单、非接触操作和低能耗而备受关注。近年来，声镊已能够对尺寸涵盖毫米级（如斑马鱼）和纳米级（如DNA）的生物靶标进行高效且智能的操控。除声镊外，其他相关的声流控芯片，包括声学分离芯片、混合芯片、富集芯片和传输芯片等，也已成为操控微/纳米生物粒子（血液中的细胞、细胞外囊泡、脂质体等）的强大平台。相应地，还开发出了一些有趣的应用，如智能传感。在本综述中，我们首先介绍声镊及各种相关技术的原理。其次，我们比较并总结了声流控技术在生物粒子操控和传感方面的最新应用。最后，我们从器件设计和跨学科等角度展望未来的发展方向。
