Department of Engineering Science and Mechanics, The Pennsylvania State University , University Park, Pennsylvania 16802, United States.
C. Eugene Bennett Department of Chemistry, West Virginia University , Morgantown, West Virginia 26506, United States.
ACS Nano. 2017 Jan 24;11(1):603-612. doi: 10.1021/acsnano.6b06784. Epub 2017 Jan 9.
Focusing and enriching submicrometer and nanometer scale objects is of great importance for many applications in biology, chemistry, engineering, and medicine. Here, we present an acoustofluidic chip that can generate single vortex acoustic streaming inside a glass capillary through using low-power acoustic waves (only 5 V is required). The single vortex acoustic streaming that is generated, in conjunction with the acoustic radiation force, is able to enrich submicrometer- and nanometer-sized particles in a small volume. Numerical simulations were used to elucidate the mechanism of the single vortex formation and were verified experimentally, demonstrating the focusing of silica and polystyrene particles ranging in diameter from 80 to 500 nm. Moreover, the acoustofluidic chip was used to conduct an immunoassay in which nanoparticles that captured fluorescently labeled biomarkers were concentrated to enhance the emitted signal. With its advantages in simplicity, functionality, and power consumption, the acoustofluidic chip we present here is promising for many point-of-care applications.
聚焦和浓缩亚微米和纳米尺度的物体对于生物学、化学、工程学和医学中的许多应用都非常重要。在这里，我们展示了一种声流控芯片，它可以通过使用低功率声波（仅需 5 V）在玻璃毛细管内产生单一的漩涡声流。所产生的单一漩涡声流与声辐射力相结合，可以在小体积内浓缩亚微米和纳米级大小的颗粒。数值模拟用于阐明单一漩涡形成的机制，并通过实验得到验证，证明了直径为 80 至 500nm 的二氧化硅和聚苯乙烯颗粒的聚焦效果。此外，该声流控芯片还用于进行免疫分析，其中捕获了荧光标记生物标志物的纳米颗粒被浓缩以增强发出的信号。由于其具有简单、功能强大和功耗低的优点，因此我们这里展示的声流控芯片有望在许多即时医疗应用中得到应用。
