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酸瓠瓜多糖的提取及其对鸡的免疫调节作用。

Extraction and immunomodulatory effects of acid Lagenaria siceraria (Molina) Standl. Polysaccharide on chickens.

机构信息

College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China; Xinjiang Key Laboratory of New Drug Study and Creation for Herbivorous Animals, Urumqi 830052, China.

Animal Husbandry and Veterinary Station, Shufu County Bureau of Agriculture and Rural Affairs, Kashgar 844100, China.

出版信息

Poult Sci. 2024 Oct;103(10):104113. doi: 10.1016/j.psj.2024.104113. Epub 2024 Jul 31.

DOI:10.1016/j.psj.2024.104113
PMID:39146923
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11379659/
Abstract

Herbal polysaccharides are extensively studied as vaccine adjuvants due to their safety and potent immunoenhancing activity. This study aimed to analyze the structure of Lagenaria siceraria (Molina) Standl polysaccharide (LSP50) and investigate its adjuvant activity for the H9N2 vaccine in broiler chickens. Structural analysis revealed that LSP50 primarily consisted of rhamnose, arabinose, xylose, mannose, glucose, and galactose with molar ratios of 23.12: 12.28: 10.87: 8.26: 2.64: 22.82 respectively. The adjuvant activity of LSP50 was evaluated, which showing significant enhancements compared to the H9N2 group. Parameters including the immune organ index, H9N2 specific IgG level, cytokines contents (IFN-γ, IL-2, IL-4, and IL-5), and the proportion of CD3eCD8aTcells were significantly increased in the LSP50 group (P < 0.05). Additionally, sequencing results showed that LSP50 modulates the immune response by regulating PLA2G12B and PTGDS genes involved in the arachidonic acid pathway. These findings were further validated through qPCR analysis to affirm the reliability of the sequencing data. In conclusion, our results demonstrate that LSP50 exhibits potent adjuvant activity, enhancing both cellular and humoral immunity.

摘要

植物多糖由于其安全性和有效的免疫增强活性，被广泛研究作为疫苗佐剂。本研究旨在分析绞股蓝多糖（LSP50）的结构，并研究其在肉鸡中作为 H9N2 疫苗佐剂的活性。结构分析表明，LSP50 主要由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖组成，摩尔比分别为 23.12:12.28:10.87:8.26:2.64:22.82。评估了 LSP50 的佐剂活性，与 H9N2 组相比，其具有显著的增强作用。LSP50 组的免疫器官指数、H9N2 特异性 IgG 水平、细胞因子含量（IFN-γ、IL-2、IL-4 和 IL-5）和 CD3eCD8aT 细胞的比例均显著升高（P < 0.05）。此外，测序结果表明，LSP50 通过调节参与花生四烯酸途径的 PLA2G12B 和 PTGDS 基因来调节免疫反应。通过 qPCR 分析进一步验证了这些发现，以确认测序数据的可靠性。总之，我们的结果表明 LSP50 具有强大的佐剂活性，增强细胞和体液免疫。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0cd/11379659/75127e17bbd8/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0cd/11379659/bff2b3ba077b/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0cd/11379659/2127a40a08b4/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0cd/11379659/cd7f5a7fb41e/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0cd/11379659/b0225e560fb1/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0cd/11379659/3d556d30f634/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0cd/11379659/c6a1bd25249b/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0cd/11379659/75127e17bbd8/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0cd/11379659/bff2b3ba077b/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0cd/11379659/2127a40a08b4/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0cd/11379659/cd7f5a7fb41e/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0cd/11379659/b0225e560fb1/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0cd/11379659/3d556d30f634/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0cd/11379659/c6a1bd25249b/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e0cd/11379659/75127e17bbd8/gr7.jpg

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