Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Science, Changsha, Hunan 410125, China.
Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China.
Genomics. 2024 Sep;116(5):110919. doi: 10.1016/j.ygeno.2024.110919. Epub 2024 Aug 13.
Ningxiang (NX) pig has been recognized as one of the most famous Chinese indigenous breeds due to its characteristics in stress resistance. However, intestinal microbial feature and gene profiling in NX piglets have not been studied. Here, we compared the intestinal microbiome and transcriptome between NX and Duroc × Landrace × Large white (DLY) piglets and found the high enrichment of several colonic Bacteroides, Prevotella and Clostridium species in NX piglets. Further functional analyses revealed their predominant function in methane, glycolysis and gluconeogenesis metabolism. Our mRNA-sequencing data unraveled the distinct colonic gene expression between these two breeds. In particular, we showed that the improved intestinal function in NX piglets may be determined by enhanced intestinal barrier gene expression and varied immune gene expression through modulating the composition of the gut microbes. Together, our study revealed the intestinal characteristics of NX piglets, providing their potential application in improving breeding strategies and developing dietary interventions.
宁乡猪因其抗应激能力而被认为是中国最著名的本土品种之一。然而，宁乡猪仔猪的肠道微生物特征和基因谱尚未得到研究。在这里，我们比较了宁乡猪和杜洛克×长白×大白（DLY）仔猪的肠道微生物组和转录组，发现宁乡猪仔猪的结肠拟杆菌、普雷沃氏菌和梭菌属的几个物种高度富集。进一步的功能分析表明，它们主要参与甲烷、糖酵解和糖异生代谢。我们的 mRNA 测序数据揭示了这两个品种之间结肠基因表达的显著差异。特别是，我们表明，通过调节肠道微生物的组成，增强肠道屏障基因表达和不同的免疫基因表达可能决定了宁乡猪仔猪改善的肠道功能。总的来说，我们的研究揭示了宁乡猪仔猪的肠道特征，为其在改善繁殖策略和开发饮食干预方面的潜在应用提供了依据。
