State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Frontiers Science Center for Animal Breeding and Sustainable Production, College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China.
Microbiome. 2023 May 8;11(1):102. doi: 10.1186/s40168-023-01551-9.
The intestinal epithelial barrier confers protection against the intestinal invasion by pathogens and exposure to food antigens and toxins. Growing studies have linked the gut microbiota to the intestinal epithelial barrier function. The mining of the gut microbes that facilitate the function of intestinal epithelial barrier is urgently needed.
Here, we studied a landscape of the gut microbiome of seven pig breeds using metagenomics and 16S rDNA gene amplicon sequencing. The results indicated an obvious difference in the gut microbiome between Congjiang miniature (CM) pigs (a native Chinese breed) and commercial Duroc × [Landrace × Yorkshire] (DLY) pigs. CM finishing pigs had stronger intestinal epithelial barrier function than the DLY finishing pigs. Fecal microbiota transplantation from CM and DLY finishing pigs to germ-free (GF) mice transferred the intestinal epithelial barrier characteristics. By comparing the gut microbiome of the recipient GF mice, we identified and validated Bacteroides fragilis as a microbial species that contributes to the intestinal epithelial barrier. B. fragilis-derived 3-phenylpropionic acid metabolite had an important function on the enhancement of intestinal epithelial barrier. Furthermore, 3-phenylpropionic acid facilitated the intestinal epithelial barrier by activating aryl hydrocarbon receptor (AhR) signaling.
These findings suggest that manipulation of B. fragilis and 3-phenylpropionic acid is a promising strategy for improving intestinal epithelial barrier. Video Abstract.
肠道上皮屏障赋予了机体抵抗肠道病原体侵袭和暴露于食物抗原和毒素的能力。越来越多的研究将肠道微生物群与肠道上皮屏障功能联系起来。迫切需要挖掘促进肠道上皮屏障功能的肠道微生物。
在这里，我们使用宏基因组学和 16S rDNA 基因扩增子测序研究了七个猪品种的肠道微生物组图谱。结果表明，中国本地品种（从江香猪，CM）和商业品种（杜洛克×[长白猪×约克夏猪]，DLY）猪之间的肠道微生物组存在明显差异。育肥期 CM 猪的肠道上皮屏障功能强于育肥期 DLY 猪。将 CM 和 DLY 育肥猪的粪便微生物群移植到无菌（GF）小鼠中，可传递肠道上皮屏障特征。通过比较受体 GF 小鼠的肠道微生物组，我们鉴定并验证了脆弱拟杆菌是促进肠道上皮屏障的微生物物种。脆弱拟杆菌衍生的 3-苯丙酸代谢物通过激活芳香烃受体（AhR）信号对增强肠道上皮屏障具有重要作用。
这些发现表明，脆弱拟杆菌和 3-苯丙酸的操纵是改善肠道上皮屏障的一种有前途的策略。视频摘要。
