Lei Linfeng, Wang Zhaobin, Li Jianzhong, Yang Huansheng, Yin Yulong, Tan Bie, Chen Jiashun
Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410125, China.
Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Animals (Basel). 2021 Jun 23;11(7):1862. doi: 10.3390/ani11071862.
Sixteen 35-day-old piglets, including eight Large White (LW) piglets (a lean-type pig breed) and eight Ningxiang (NX) piglets (a fatty-type Chinese Indigenous pig breed), were fed the same diet for 105 days. NX pigs had higher intramuscular fat content than LW pigs ( < 0.05). According to 16S rRNA gene sequencing, the relative abundances of the genera Ruminococcaceaegroup, Christensenellaaceaegroup and were higher, whereas the abundances of Prevotellaceae_group, , and were lower, in the colon of NX pigs compared to that of LW pigs. Nonmetric multidimensional scaling analysis revealed that the microbiota of the two pig breeds clustered separately along the principal coordinate axis. Furthermore, functional prediction of the bacterial communities suggested higher fatty acid biosynthesis in NX pigs. NX pigs also exhibited lower concentrations of total short-chain fatty acids, propionate and butyrate in the colon ( < 0.05). These findings suggest that NX pigs exhibited higher intramuscular fat content and backfat thickness than LW pigs. The bacterial communities in the colon of NX pigs were also more diverse than those in the colon of LW pigs, which might be used as a potential metabolomics mechanism to research different breeds of pigs.
16头35日龄仔猪，包括8头大白猪（LW，一种瘦肉型猪品种）和8头宁乡猪（NX，一种脂肪型中国本土猪品种），用相同日粮饲喂105天。宁乡猪的肌内脂肪含量高于大白猪（P<0.05）。根据16S rRNA基因测序，与大白猪相比，宁乡猪结肠中瘤胃球菌科菌群、克里斯滕森菌科菌群等属的相对丰度较高，而普雷沃氏菌科菌群、……等的丰度较低。非度量多维尺度分析表明，两个猪品种的微生物群沿主坐标轴分别聚类。此外，细菌群落的功能预测表明宁乡猪的脂肪酸生物合成较高。宁乡猪结肠中总短链脂肪酸、丙酸和丁酸的浓度也较低（P<0.05）。这些发现表明，宁乡猪的肌内脂肪含量和背膘厚度高于大白猪。宁乡猪结肠中的细菌群落也比大白猪结肠中的细菌群落更多样化，这可能作为研究不同猪品种的潜在代谢组学机制。
