Environmental and Radiation Health Sciences Directorate, Health Canada, 775 Brookfield Road, PL 6303B, Ottawa, ON K1A 1C1, Canada.
Environmental and Radiation Health Sciences Directorate, Health Canada, 775 Brookfield Road, PL 6303B, Ottawa, ON K1A 1C1, Canada; University of Ottawa, Ottawa Hospital Research Institute, 501 Smyth Road, Ottawa, ON K1H 8L6, Canada.
Mutat Res Genet Toxicol Environ Mutagen. 2024 Aug-Sep;898:503792. doi: 10.1016/j.mrgentox.2024.503792. Epub 2024 Jul 5.
In the event of a large-scale incident involving radiological or nuclear exposures, there is a potential for large numbers of individuals to have received doses of radiation sufficient to cause adverse health effects. It is imperative to quickly identify these individuals in order to provide information to the medical community to assist in making decisions about their treatment. The cytokinesis-block micronucleus assay is a well-established method for performing biodosimetry. This assay has previously been adapted to imaging flow cytometry and has been validated as a high-throughput option for providing dose estimates in the range of 0-10 Gy. The goal of this study was to test the ability to further optimize the assay by reducing the time of culture to 48 h from 68 h as well as reducing the volume of blood required for the analysis to 200 μL from 2 mL. These modifications would provide efficiencies in time and ease of processing impacting the ability to manage large numbers of samples and provide dose estimates in a timely manner. Results demonstrated that either the blood volume or the culture time could be reduced while maintaining dose estimates with sufficient accuracy for triage analysis. Reducing both the blood volume and culture time, however, resulted in poor dose estimates. In conclusion, depending on the needs of the scenario, either culture time or the blood volume could be reduced to improve the efficiency of analysis for mass casualty scenarios.
如果发生涉及放射性或核辐射暴露的大规模事件，可能会有大量个体受到足以引起健康不良影响的辐射剂量。必须迅速确定这些个体，以便向医疗界提供信息，协助决定对其进行治疗。有丝分裂阻滞微核试验是进行生物剂量测定的一种成熟方法。该试验先前已被改编为成像流式细胞术，并已被验证为提供 0-10Gy 范围内剂量估计的高通量选择。本研究的目的是测试通过将培养时间从 68 小时缩短至 48 小时以及将分析所需的血液量从 2 毫升减少至 200 微升来进一步优化该试验的能力。这些修改将在时间和处理便利性方面提供效率，从而影响管理大量样本和及时提供剂量估计的能力。结果表明，在保持用于分诊分析的足够准确性的情况下，可以减少血液量或培养时间。然而，同时减少血液量和培养时间会导致剂量估计不佳。总之，根据情况的需要，可以减少培养时间或血液量，以提高大规模伤亡情况下的分析效率。
