用协同凝集试验与GM1-ELISA及Y-1肾上腺细胞试验检测大肠杆菌不耐热肠毒素的比较
Comparison of a coagglutination test with the GM1-ELISA and the Y-1 adrenal cell assay for the detection of heat-labile enterotoxin from Escherichia coli.
作者信息
Steinrück H, Samtleben S, Rönnberg B, Wadström T
出版信息
Zentralbl Bakteriol Mikrobiol Hyg A. 1985 Dec;260(4):436-42. doi: 10.1016/s0176-6724(85)80064-x.
A number of different assay methods has been developed for the detection of the heat-labile enterotoxin (LT) of ETEC strains isolated from humans and animals. In the present study 40 Escherichia coli strains were subject of a comparative study of the staphylococcal coagglutination (Coa-LT) test, the GM1-ELISA and the Y-1 adrenal cell test. All but two of 20 "LT-ST" or "LT-only" producing ETEC strains gave identical results in both the Coa-LT test and the bioassay. None of the 14 "ST only" producing ETEC strains gave false positive results. It is concluded that the Coa-LT test is a simple and rapid assay method for the routine diagnosis of LT producing ETEC strains.
已经开发出多种不同的检测方法来检测从人和动物中分离出的肠毒素大肠杆菌（ETEC）菌株的不耐热肠毒素（LT）。在本研究中，对40株大肠杆菌菌株进行了葡萄球菌协同凝集（Coa-LT）试验、GM1-ELISA和Y-1肾上腺细胞试验的比较研究。20株产生“LT-ST”或“仅LT”的ETEC菌株中，除两株外，其余在Coa-LT试验和生物测定中均给出相同结果。14株仅产生“ST”的ETEC菌株均未给出假阳性结果。得出结论，Coa-LT试验是用于常规诊断产生LT的ETEC菌株的一种简单快速的检测方法。
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