Sen D, Saha M R, Pal S C
J Clin Microbiol. 1984 Feb;19(2):194-6. doi: 10.1128/jcm.19.2.194-196.1984.
Three simple immunological tests, the modified Elek (Biken) test, the modified staphylococcal coagglutination test, and the rapid GM1-horseradish peroxidase-enzyme-linked immunosorbent assay have been evaluated for detection of heat-labile enterotoxin of enterotoxigenic Escherichia coli. Of the 100 coded E. coli strains tested, 94 gave consistent results with all the three immunological tests; a discrepancy was observed in only 6 strains. Identical results were obtained when the Biken test was conducted with complete and incomplete Biken kits (Meguro Institute Ltd., Osaka, Japan). All three immunological tests evaluated in this study were found to be sensitive and simple and can be easily adopted by any laboratory for detection of heat-labile enterotoxins of enterotoxigenic E. coli strains.
已对三种简单的免疫检测方法，即改良Elek（比肯）试验、改良葡萄球菌凝聚试验和快速GM1-辣根过氧化物酶-酶联免疫吸附测定法进行了评估，以检测产肠毒素大肠杆菌的不耐热肠毒素。在所检测的100株编码大肠杆菌菌株中，94株在所有三种免疫检测中结果一致；仅6株出现差异。使用完整和不完整的比肯试剂盒（日本大阪目黑研究所有限公司）进行比肯试验时，得到了相同的结果。本研究中评估的所有三种免疫检测方法均灵敏且操作简单，可以被任何实验室轻松采用，用于检测产肠毒素大肠杆菌菌株的不耐热肠毒素。
