Department of Virology, Ministry of Higher Education and Scientific Research, Khartoum, Sudan.
National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
F1000Res. 2024 Oct 23;11:1401. doi: 10.12688/f1000research.123652.3. eCollection 2022.
Diarrhea is a significant health problem in the Third World. Identification of the pathogen that causes diarrhea is vital for measures to prevent and control this disease. There are also very few reports of diarrhea in Sudan. Our study aimed to determine the Prevalence of specific protozoan pathogens ( , , and ) in children in Khartoum, Sudan.
We conducted a cross-sectional survey among children under five years of age hospitalized with acute diarrhea between April and December 2014. Diarrheal stool samples were collected, and , , and were examined using multiplex real-time PCR.
Four hundred and thirty-seven children with acute diarrhea were included in this study; the higher prevalence of diarrhea was in the age ≤ 2 years old (403, 92.2%), >2-≤4 years (32, 7.3%), and >4-<5 years (2, 0.5%). The male-to-female ratio in this study was 1:1.7. Infection with intestinal parasite was found in 155 (35.5%) cases, and co-infection was detected in 16 (3.7%) cases. (18.8%) and (15.8%) were the most frequently identified parasites, followed by (0.9). The parasite infection rate was highest and lowest in the under 2-year-old group 143 (35.5%) and the 2-4-year-old group 12 (37.5%). The infection rate was higher in boys 104 (37.7%) than in girls 51 (31.7%). The number of positive cases was higher in the rainy season (August to December) 143 (37.4%), corresponding with that in the dry Season (April to June) 12 (21.8%).
Our present study demonstrated the high prevalence of and in children with diarrhea in the Khartoum region and the usefulness of the multiplex real-time method in disclosing pathogenic protozoal agents. Our result highlighted the necessity of developing intervention measurement and control strategies to deal with childhood parasitic diarrhea in this region.
腹泻是第三世界的一个重大健康问题。确定导致腹泻的病原体对于预防和控制这种疾病的措施至关重要。苏丹也很少有腹泻的报告。我们的研究旨在确定苏丹喀土穆地区五岁以下儿童中特定原生动物病原体（ 、 、 ）的患病率。
我们对 2014 年 4 月至 12 月期间因急性腹泻住院的 5 岁以下儿童进行了横断面调查。采集腹泻粪便样本，使用多重实时 PCR 检测 、 、 。
本研究共纳入 437 例急性腹泻患儿；年龄≤2 岁（403 例，92.2%）、2-≤4 岁（32 例，7.3%）和>4-<5 岁（2 例，0.5%）腹泻发生率较高。本研究中男女性别比为 1:1.7。155 例（35.5%）患儿感染肠道寄生虫，16 例（3.7%）患儿合并感染。 （18.8%）和 （15.8%）是最常鉴定的寄生虫，其次是 （0.9%）。2 岁以下组感染率最高，为 143 例（35.5%），2-4 岁组感染率最低，为 12 例（37.5%）。男孩感染率（37.7%）高于女孩（31.7%），感染率在男孩中为 104 例，在女孩中为 51 例。阳性病例数在雨季（8 月至 12 月）较多，为 143 例（37.4%），与旱季（4 月至 6 月）的 12 例（21.8%）相对应。
本研究表明，喀土穆地区儿童腹泻中 和 的患病率较高，多重实时方法在揭示致病原生动物因子方面具有实用性。我们的研究结果强调了在该地区制定干预措施和控制策略以应对儿童寄生虫性腹泻的必要性。
