Liu Xue, Tan Yonghong, Zhang Jianyu, Huang Weigeng, Yan Dingyuan, Wang Dong, Tang Ben Zhong
Center for AIE Research, Shenzhen Key Laboratory of Polymer Science and Technology, Guangdong Research Center for Interfacial Engineering of Functional Materials, College of Materials Science and Engineering, Shenzhen University Shenzhen 518060 China
Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Department of Chemistry, State Key Laboratory of Neuroscience, Department of Chemical and Biomedical Engineering, The Hong Kong University of Science and Technology Kowloon Hong Kong 999077 China.
Chem Sci. 2024 Jul 15;15(32):12957-12963. doi: 10.1039/d4sc01721h. eCollection 2024 Aug 14.
Concurrent near-infrared-II (NIR-II) fluorescence imaging (FLI) and photoacoustic imaging (PAI) holds tremendous potential for effective disease diagnosis owing to their combined benefits and complementary features, in particular on the basis of a single molecule. However, the simultaneous guarantee of high-quality NIR-II FLI and PAI is recognized to be challenging impeded by the competitive photophysical processes at the molecular level. Herein, a simple organic fluorophore, namely T-NSD, is finely engineered with facile synthetic procedures through delicately modulating the rigidity and electron-withdrawing ability of the molecular acceptor. The notable advantages of fabricated T-NSD nanoparticles include a large Stokes shift, intense fluorescence emission in the NIR-II region, and anti-quenching properties in the aggregated states, which eventually enable the implementation of dual-modal NIR-II FLI/PAI in a 4T1 tumor-xenografted mouse model with reliable performance and good biocompatibility. Overall, these findings present a simple strategy for the construction of NIR-II optical agents to allow multimodal disease diagnosis.
由于其综合优势和互补特性，特别是基于单一分子，同时进行近红外二区（NIR-II）荧光成像（FLI）和光声成像（PAI）在有效疾病诊断方面具有巨大潜力。然而，在分子水平上，竞争性的光物理过程阻碍了同时保证高质量的NIR-II FLI和PAI，这被认为具有挑战性。在此，通过精心调节分子受体的刚性和吸电子能力，采用简便的合成方法对一种简单的有机荧光团T-NSD进行了精细设计。制备的T-NSD纳米颗粒的显著优点包括大的斯托克斯位移、在NIR-II区域强烈的荧光发射以及聚集态下的抗猝灭特性，最终使得在4T1肿瘤异种移植小鼠模型中实现双模态NIR-II FLI/PAI成为可能，其性能可靠且生物相容性良好。总体而言，这些发现为构建用于多模态疾病诊断的NIR-II光学试剂提供了一种简单策略。
