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从（罗克斯伯）G. 唐松针中分离、表征及液相色谱 - 串联质谱法测定抗肥胖成分

Isolation, characterization, and LC MS/MS determination of anti-obesity components from pine needles of (Roxb.) G. Don.

作者信息

Wang Xin, Li Bin, Liu Dongyan, Guo Yuer, Zhang Jiaxu, Li Wanyu, Peng Tengteng, Ma Quhuan, Shi Xiaofeng

机构信息

College of Pharmacy, Gansu University of Traditional Chinese Medicine, Lanzhou, China.

Institute of Pharmaceutical research, Gansu Academy of Medical Sciences, Lanzhou, China.

出版信息

Front Nutr. 2024 Aug 1;11:1448908. doi: 10.3389/fnut.2024.1448908. eCollection 2024.

DOI:10.3389/fnut.2024.1448908
PMID:39149547
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11324603/
Abstract

OBJECTIVE

This study aimed to isolate and analyze the components in cedar pine needles (needle leaves of (Roxb.) G. Don) that exhibit anti-obesity effects, as determined through animal experiments.

METHODS

The extract of cedar pine needles was separated into four fractions of different polarities using a macroporous resin column. The fraction that retained anti-obesity activity was evaluated based on the results of animal experiments. Monomeric compounds were structurally characterized and isolated from the active fraction using a preparative liquid chromatography system. Combined with subsequent glucose gel chromatographic separation. The content of the separated components was determined using ultrahigh performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-QQQ-MS/MS).

RESULTS

The water-washed fraction retained anti-obesity activity of the cedar pine needles more effectively. A total of 16 compounds were separated from this fraction, and the contents of 14 of these compounds were determined to be present in cedar pine needles.

CONCLUSION

Nine components, namely p-hydroxy benzyl alcohol, chlorogenic acid, vanillic acid, syringic acid, P-coumaric acid, sinapic acid, benzoic acid, phenylacetic acid, salicylic acid, were characterized and determined for the first time in cedar pine needles. The components with anti-obesity activity in the pine needles of are mainly derived from phenolic acids.

摘要

目的

本研究旨在通过动物实验，分离并分析雪松松针（(Roxb.) G. Don的针叶）中具有抗肥胖作用的成分。

方法

采用大孔树脂柱将雪松松针提取物分离成四个不同极性的馏分。根据动物实验结果评估保留抗肥胖活性的馏分。使用制备液相色谱系统从活性馏分中对单体化合物进行结构表征和分离。结合后续的葡聚糖凝胶色谱分离。使用超高效液相色谱-三重四极杆质谱联用仪（UPLC-QQQ-MS/MS）测定分离成分的含量。

结果

水洗馏分更有效地保留了雪松松针的抗肥胖活性。从该馏分中总共分离出16种化合物，其中14种化合物的含量在雪松松针中被测定。

结论

对羟基苯甲醇、绿原酸、香草酸、丁香酸、对香豆酸、芥子酸、苯甲酸、苯乙酸、水杨酸这9种成分在雪松松针中首次被表征和测定。雪松松针中具有抗肥胖活性的成分主要来源于酚酸。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a0/11324603/dda92e17f1b1/fnut-11-1448908-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a0/11324603/40e83f7b12ba/fnut-11-1448908-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a0/11324603/5c30f61241c0/fnut-11-1448908-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a0/11324603/69725f095af4/fnut-11-1448908-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a0/11324603/97a98aa25027/fnut-11-1448908-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a0/11324603/dda92e17f1b1/fnut-11-1448908-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a0/11324603/40e83f7b12ba/fnut-11-1448908-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a0/11324603/5c30f61241c0/fnut-11-1448908-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a0/11324603/69725f095af4/fnut-11-1448908-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a0/11324603/97a98aa25027/fnut-11-1448908-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6a0/11324603/dda92e17f1b1/fnut-11-1448908-g004.jpg

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