State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China.
Gansu Engineering Research Center of Medical Collagen, Lanzhou 730000, P. R. China.
Biomater Sci. 2024 Sep 25;12(19):4946-4956. doi: 10.1039/d4bm00860j.
Skin damage caused by excessive UV exposure has gradually become one of the most common skin diseases, leading to desquamation, scab formation, inflammation and even skin cancer. Animal-derived hydrolyzed collagen peptides have been developed to treat UV-damaged skin; however, they have raised severe concerns such as potential viral transmission, random sequences and the lack of a triple helix structure. Nano collagen, a novel type of short collagen, has attracted increasing attention in the mimicking of natural collagen, while its applications in UV-damaged skin treatment remains unexplored. Herein, we have created a series of nano collagens and for the first time studied their capability of accelerating UV-damaged skin healing. Nano collagens, consisting of repetitive (GPO)n triplets and a GFOGER motif, display a stable triple-helical structure, significantly promoting fibroblast adhesion, proliferation, and migration. The repair effects of nano collagens have been investigated using an acute UV-damaged skin mouse model. Combo evaluations indicate that nano collagens contribute to recovering the dermis density and erythema index of UV-damaged skin. Histological analysis further demonstrates their capability of promoting the healing of damaged skin by accelerating re-epithelialization and collagen regeneration. These highly bioactive triple-helical nano collagens present a novel strategy for the treatment of UV-damaged skin, providing promising applications in cosmetics and dermatology.
过度紫外线照射导致的皮肤损伤逐渐成为最常见的皮肤疾病之一，导致脱皮、结痂、炎症，甚至皮肤癌。已经开发出动物源性水解胶原蛋白肽来治疗紫外线损伤皮肤；然而，它们引发了严重的问题，如潜在的病毒传播、随机序列和缺乏三螺旋结构。新型短胶原纳米胶原在模拟天然胶原方面引起了越来越多的关注，但其在治疗紫外线损伤皮肤方面的应用仍有待探索。在此，我们制备了一系列纳米胶原，并首次研究了它们加速紫外线损伤皮肤愈合的能力。由重复 (GPO)n 三肽和 GFOGER 基序组成的纳米胶原具有稳定的三螺旋结构，显著促进成纤维细胞的黏附、增殖和迁移。使用急性紫外线损伤皮肤小鼠模型研究了纳米胶原的修复效果。综合评价表明，纳米胶原有助于恢复紫外线损伤皮肤的真皮密度和红斑指数。组织学分析进一步表明，纳米胶原通过加速再上皮化和胶原再生来促进损伤皮肤的愈合。这些具有高度生物活性的三螺旋纳米胶原为治疗紫外线损伤皮肤提供了一种新策略，在化妆品和皮肤病学领域具有广阔的应用前景。
