Key Laboratory of Smart Breeding (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Tianjin Agricultural University, Tianjin, China.
Tianjin Key Laboratory of Aqua-ecology and Aquaculture, Fisheries College, Tianjin Agricultural University, Tianjin, China.
Commun Biol. 2024 Aug 17;7(1):1006. doi: 10.1038/s42003-024-06727-z.
Antibiotic-induced dysbiosis in the fish gut causes significant adverse effects. We use fecal microbiota transplantation (FMT) to accelerate the restoration of florfenicol-perturbed intestinal microbiota in koi carp, identifying key bacterial populations and metabolites involved in the recovery process through microbiome and metabolome analyses. We demonstrate that florfenicol disrupts intestinal microbiota, reducing beneficial genera such as Lactobacillus, Bifidobacterium, Bacteroides, Romboutsia, and Faecalibacterium, and causing mucosal injuries. Key metabolites, including aromatic amino acids and glutathione-related compounds, are diminished. We show that FMT effectively restores microbial populations, repairs intestinal damage, and normalizes critical metabolites, while natural recovery is less effective. Spearman correlation analyses reveal strong associations between the identified bacterial genera and the levels of aromatic amino acids and glutathione-related metabolites. This study underscores the potential of FMT to counteract antibiotic-induced dysbiosis and maintain fish intestinal health. The restored microbiota and normalized metabolites provide a basis for developing personalized probiotic therapies for fish.
抗生素诱导的鱼类肠道菌群失调会产生重大的不良影响。我们采用粪便微生物群移植（FMT）来加速恢复氟苯尼考扰乱的锦鲤肠道微生物群，通过微生物组和代谢组分析，确定了恢复过程中涉及的关键细菌种群和代谢物。研究表明，氟苯尼考会破坏肠道微生物群，减少乳酸杆菌、双歧杆菌、拟杆菌、罗姆布茨菌和粪杆菌等有益菌的数量，并导致黏膜损伤。关键代谢物，包括芳香族氨基酸和谷胱甘肽相关化合物，也会减少。我们发现 FMT 可有效恢复微生物种群、修复肠道损伤并使关键代谢物恢复正常，而自然恢复效果较差。Spearman 相关性分析表明，所鉴定的细菌属与芳香族氨基酸和谷胱甘肽相关代谢物的水平之间存在很强的关联。这项研究强调了 FMT 对抗生素诱导的菌群失调和维持鱼类肠道健康的潜力。恢复的微生物群和正常的代谢物为开发鱼类个性化益生菌疗法提供了基础。
