Sports Medicine Department of The Sixth Affiliated Hospital of Xinjiang Medical University, No.39, Wuxing South Road, Urumqi City, 830000, China.
Oncology Department of The First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830000, China.
J Orthop Surg Res. 2024 Aug 17;19(1):487. doi: 10.1186/s13018-024-04959-7.
Osteoporosis (OP) is a high-incidence bone disease that is prone to osteoporotic fractures (OF), so it has attracted widespread attention.
This study investigated the specific expression and role of miR-331 in patients with OP and OF. The findings have profound implications for the clinical prevention and treatment of these conditions.
The study included 60 OP patients, 46 OF patients, and 40 healthy controls. The expression level of miR-331-3p was detected using RT-qPCR. BMP2 was used to stimulate differentiation in MC3T3-E1 cells. After induction, the expression activity of osteogenic differentiation-related gene markers was detected using RT-qPCR. The target gene analysis was conducted using a luciferase reporter assay.
The levels of miR-331-3p were significantly elevated, while NRP2 levels were significantly reduced in OF patients. Post-surgery, miR-331-3p levels decreased over time. MiR-331-3p was found to negatively regulate the luciferase activity of NPR2 in MC3T3-E1 cells. Furthermore, overexpression of miR-331-3p inhibited cell proliferation and decreased the levels of osteoblast differentiation markers.
The up-regulation of miR-331-3p can promote OP and might also encourage the occurrence of OF by regulating NRP2. However, this needs further verification.
骨质疏松症（OP）是一种高发的骨骼疾病，容易发生骨质疏松性骨折（OF），因此引起了广泛关注。
本研究旨在探讨 miR-331 在 OP 和 OF 患者中的具体表达和作用。研究结果对这些疾病的临床预防和治疗具有深远意义。
该研究纳入了 60 名 OP 患者、46 名 OF 患者和 40 名健康对照者。采用 RT-qPCR 检测 miR-331-3p 的表达水平。用 BMP2 刺激 MC3T3-E1 细胞分化。诱导后，采用 RT-qPCR 检测成骨分化相关基因标志物的表达活性。采用荧光素酶报告基因检测进行靶基因分析。
OF 患者 miR-331-3p 水平显著升高，NRP2 水平显著降低。术后 miR-331-3p 水平随时间推移而降低。miR-331-3p 被发现可负调控 MC3T3-E1 细胞中 NPR2 的荧光素酶活性。此外，miR-331-3p 的过表达抑制细胞增殖，并降低成骨细胞分化标志物的水平。
miR-331-3p 的上调可促进 OP 的发生，可能通过调节 NRP2 而促进 OF 的发生。然而，这需要进一步验证。
