Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, MB, Canada; Children's Hospital Research Institute of Manitoba, Winnipeg, MB, Canada.
Children's Hospital Research Institute of Manitoba, Winnipeg, MB, Canada; Manitoba Chemosensory Biology Research Group, Department of Oral Biology, University of Manitoba, Winnipeg, MB, Canada; Department of Preventive Dental Science, University of Manitoba, Winnipeg, MB, Canada.
Cell Rep. 2024 Aug 27;43(8):114635. doi: 10.1016/j.celrep.2024.114635. Epub 2024 Aug 17.
Early childhood caries (ECC) is influenced by microbial and host factors, including social, behavioral, and oral health. In this cross-sectional study, we analyze interkingdom dynamics in the dental plaque microbiome and its association with host variables. We use 16S rRNA and ITS1 amplicon sequencing on samples collected from preschool children and analyze questionnaire data to examine the social determinants of oral health. The results indicate a significant enrichment of Streptococcus mutans and Candida dubliniensis in ECC samples, in contrast to Neisseria oralis in caries-free children. Our interkingdom correlation analysis reveals that Candida dubliniensis is strongly correlated with both Neisseria bacilliformis and Prevotella veroralis in ECC. Additionally, ECC shows significant associations with host variables, including oral health status, age, place of residence, and mode of childbirth. This study provides empirical evidence associating the oral microbiome with socioeconomic and behavioral factors in relation to ECC, offering insights for developing targeted prevention strategies.
婴幼儿龋（ECC）受微生物和宿主因素的影响，包括社会、行为和口腔健康。在这项横断面研究中，我们分析了牙菌斑微生物组中的跨界动态及其与宿主变量的关联。我们使用来自学龄前儿童的样本进行 16S rRNA 和 ITS1 扩增子测序，并分析问卷调查数据，以研究口腔健康的社会决定因素。结果表明，ECC 样本中显著富集了变异链球菌和都柏林假丝酵母，而无龋儿童中则富含口腔奈瑟菌。我们的跨界相关性分析表明，ECC 中假丝酵母与奈瑟菌和小韦荣球菌呈强相关性。此外，ECC 与宿主变量（包括口腔健康状况、年龄、居住地和分娩方式）显著相关。本研究提供了与 ECC 相关的口腔微生物组与社会经济和行为因素的实证证据，为制定有针对性的预防策略提供了思路。
