Tangchitphisut P, Srikaew N, Numhom S, Tangprasittipap A, Woratanarat P, Wongsak S, Kijkunasathian C, Hongeng S, Murray I R, Tawonsawatruk T
Department of Orthopaedics, Faculty of Medicine, Ramathibodi Hospital, Bangkok 10400, Thailand.
Office of Research and Innovation, Ramathibodi Hospital, Bangkok 10400, Thailand.
Arthritis. 2016;2016:4019873. doi: 10.1155/2016/4019873. Epub 2016 Apr 26.
Introduction. The Infrapatellar fat pad (IPFP) represents an emerging alternative source of adipose-derived mesenchymal stem cells (ASCs). We compared the characteristics and differentiation capacity of ASCs isolated from IPFP and SC. Materials and Methods. ASCs were harvested from either IPFP or SC. IPFPs were collected from patients undergoing total knee arthroplasty (TKA), whereas subcutaneous tissues were collected from patients undergoing lipoaspiration. Immunophenotypes of surface antigens were evaluated. Their ability to form colony-forming units (CFUs) and their differentiation potential were determined. The ASCs karyotype was evaluated. Results. There was no difference in the number of CFUs and size of CFUs between IPFP and SC sources. ASCs isolated from both sources had a normal karyotype. The mesenchymal stem cells (MSCs) markers on flow cytometry was equivalent. IPFP-ASCs demonstrated significantly higher expression of SOX-9 and RUNX-2 over ASCs isolated from SC (6.19 ± 5.56-, 0.47 ± 0.62-fold; p value = 0.047, and 17.33 ± 10.80-, 1.56 ± 1.31-fold; p value = 0.030, resp.). Discussion and Conclusion. CFU assay of IPFP-ASCs and SC-ASCs harvested by lipoaspiration technique was equivalent. The expression of key chondrogenic and osteogenic genes was increased in cells isolated from IPFP. IPFP should be considered a high quality alternative source of ASCs.
引言。髌下脂肪垫（IPFP）是脂肪间充质干细胞（ASC）的一种新兴替代来源。我们比较了从IPFP和皮下组织（SC）分离的ASC的特征和分化能力。材料与方法。ASC分别从IPFP或SC中获取。IPFP取自接受全膝关节置换术（TKA）的患者，而皮下组织取自接受抽脂术的患者。评估表面抗原的免疫表型。测定它们形成集落形成单位（CFU）的能力及其分化潜能。评估ASC的核型。结果。IPFP和SC来源的CFU数量和CFU大小没有差异。从这两个来源分离的ASC核型正常。流式细胞术检测的间充质干细胞（MSC）标志物相当。与从SC分离的ASC相比，IPFP-ASC中SOX-9和RUNX-2的表达显著更高（分别为6.19±5.56倍、0.47±0.62倍；p值 = 0.047，以及17.33±10.80倍、1.56±1.31倍；p值 = 0.030）。讨论与结论。通过抽脂技术获取的IPFP-ASC和SC-ASC的CFU检测相当。从IPFP分离的细胞中关键软骨生成和成骨基因的表达增加。IPFP应被视为ASC的高质量替代来源。
