Chaleff D T, Tatchell K
Mol Cell Biol. 1985 Aug;5(8):1878-86. doi: 10.1128/mcb.5.8.1878-1886.1985.
In the yeast Saccharomyces cerevisiae, haploid cells occur in one of the two cell types, a or alpha. The allele present at the mating type (MAT) locus plays a prominent role in the control of cell type expression. An important consequence of the elaboration of cell type is the ability of cells of one mating type to conjugate with cells of the opposite mating type, resulting in yet a third cell type, an a/alpha diploid. Numerous genes that are involved in the expression of cell type and the conjugation process have been identified by standard genetic techniques. Molecular analysis has shown that expression of several of these genes is subject to control on the transcriptional level by the MAT locus. Two genes, STE7 and STE11, are required for mating in both haploid cell types; ste7 and ste11 mutants are sterile. We report here the molecular cloning of STE7 and STE11 genes and show that expression of these genes is not regulated transcriptionally by the MAT locus. We also have genetically mapped the STE11 gene to chromosome XII, 40 centimorgans from ura4.
在酿酒酵母中，单倍体细胞有两种细胞类型之一，即a型或α型。位于交配型（MAT）位点的等位基因在细胞类型表达的控制中起着重要作用。细胞类型分化的一个重要结果是一种交配型的细胞能够与相反交配型的细胞结合，产生第三种细胞类型，即a/α二倍体。通过标准遗传技术已经鉴定出许多参与细胞类型表达和结合过程的基因。分子分析表明，这些基因中的几个基因的表达在转录水平上受MAT位点的控制。两个基因STE7和STE11是两种单倍体细胞类型交配所必需的；ste7和ste11突变体是不育的。我们在此报告STE7和STE11基因的分子克隆，并表明这些基因的表达不受MAT位点的转录调控。我们还通过遗传方法将STE11基因定位到第十二号染色体上，距离ura4基因40厘摩。
