Laloux I, Dubois E, Dewerchin M, Jacobs E
Labatoire de Microbiologie, Faculté des Sciences, Université Libre de Bruxelles, Belgium.
Mol Cell Biol. 1990 Jul;10(7):3541-50. doi: 10.1128/mcb.10.7.3541-3550.1990.
Ty and Ty-mediated gene expression observed in haploid cells of Saccharomyces cerevisiae depends on several determinants, some of which are required for the expression of haploid-specific genes. We report here the cloning and molecular analysis of TEC1. TEC1 encodes a 486-amino-acid protein that is a trans-acting factor required for full Ty1 expression and Ty1-mediated gene activation. However, mutation or deletion of the TEC1 gene had little effect on total Ty2 transcript levels. Our analysis provides clear evidence that TEC1 is not involved in mating or sporulation processes. Unlike most of the proteins involved in Ty and adjacent gene expression, the product of TEC1 has no known cellular function. Although there was no mating-type effect on TEC1 expression, our results indicate that the TEC1 and the a/alpha diploid controls on Ty1 expression are probably not cumulative.
在酿酒酵母单倍体细胞中观察到的Ty及Ty介导的基因表达取决于几个决定因素，其中一些是单倍体特异性基因表达所必需的。我们在此报告TEC1的克隆及分子分析。TEC1编码一个486个氨基酸的蛋白质，它是Ty1充分表达及Ty1介导的基因激活所必需的反式作用因子。然而，TEC1基因的突变或缺失对Ty2转录本的总水平影响很小。我们的分析提供了明确的证据，表明TEC1不参与交配或孢子形成过程。与大多数参与Ty及相邻基因表达的蛋白质不同，TEC1的产物没有已知的细胞功能。尽管TEC1的表达没有交配型效应，但我们的结果表明，TEC1与a/α二倍体对Ty1表达的控制可能不是累加的。
