Department of Plastic and Reconstructive Surgery, Department of Cardiology, Shanghai Key Lab of Tissue Engineering, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, P. R. China.
College of Textiles, State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, 201620, P. R. China.
Adv Sci (Weinh). 2024 Oct;11(39):e2405420. doi: 10.1002/advs.202405420. Epub 2024 Aug 19.
Functional segmental trachea reconstruction is a critical concern in thoracic surgery, and tissue-engineered trachea (TET) holds promise as a potential solution. However, current TET falls short in fully restoring physiological function due to the lack of the intricate multi-tissue structure found in natural trachea. In this research, a multi-tissue integrated tissue-engineered trachea (MI-TET) is successfully developed by orderly assembling various cells (chondrocytes, fibroblasts and epithelial cells) on 3D-printed PGS bioelastomer scaffolds. The MI-TET closely resembles the complex structures of natural trachea and achieves the integrated regeneration of four essential tracheal components: C-shaped cartilage ring, O-shaped vascularized fiber ring, axial fiber bundle, and airway epithelium. Overall, the MI-TET demonstrates highly similar multi-tissue structures and physiological functions to natural trachea, showing promise for future clinical advancements in functional TETs.
功能性节段性气管重建是胸外科的一个关键关注点，组织工程气管（TET）有望成为一种潜在的解决方案。然而，由于缺乏天然气管中存在的复杂多组织结构，目前的 TET 在完全恢复生理功能方面存在不足。在这项研究中，通过在 3D 打印的 PGS 生物弹性体支架上有序组装各种细胞（软骨细胞、成纤维细胞和上皮细胞），成功开发了一种多组织整合的组织工程气管（MI-TET）。MI-TET 与天然气管的复杂结构非常相似，实现了四个重要气管成分的综合再生：C 形软骨环、O 形血管化纤维环、轴向纤维束和气道上皮。总体而言，MI-TET 表现出与天然气管非常相似的多组织结构和生理功能，有望在未来的功能性 TET 临床进展中取得突破。
