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载有 BMSCs-exos 的 GMOCS 水凝胶推进骨关节炎治疗。

Advancing osteoarthritis therapy with GMOCS hydrogel-loaded BMSCs-exos.

机构信息

Department of Orthopedics, The First Hospital of China Medical University, No. 155 Nanjing North Street, Shenyang, 110001, Liaoning Province, China.

出版信息

J Nanobiotechnology. 2024 Aug 19;22(1):493. doi: 10.1186/s12951-024-02713-z.

DOI:10.1186/s12951-024-02713-z
PMID:39160590
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11334447/
Abstract

This study investigated the mechanism of the extracellular matrix-mimicking hydrogel-mediated TGFB1/Nrf2 signaling pathway in osteoarthritis using bone marrow mesenchymal stem cell-derived exosomes (BMSCs-Exos). A GMOCS-Exos hydrogel was synthesized and evaluated for its impact on chondrocyte viability and neutrophil extracellular traps (NETs) formation. In an OA rat model, GMOCS-Exos promoted cartilage regeneration and inhibited NETs formation. Transcriptome sequencing identified TGFB1 as a key gene, with GMOCS-Exos activating Nrf2 signaling through TGFB1. Depletion of TGFB1 hindered the cartilage-protective effect of GMOCS-Exos. This study sheds light on a promising therapeutic strategy for osteoarthritis through GMOCS-Exos-mediated TGFB1/Nrf2 pathway modulation.

摘要

本研究使用骨髓间充质干细胞来源的外泌体（BMSCs-Exos）探讨细胞外基质模拟水凝胶介导的 TGFB1/Nrf2 信号通路在骨关节炎中的作用机制。合成了 GMOCS-Exos 水凝胶，并评估其对软骨细胞活力和中性粒细胞胞外陷阱（NETs）形成的影响。在 OA 大鼠模型中，GMOCS-Exos 促进软骨再生并抑制 NETs 形成。转录组测序鉴定出 TGFB1 是一个关键基因，GMOCS-Exos 通过 TGFB1 激活 Nrf2 信号通路。TGFB1 的耗竭会阻碍 GMOCS-Exos 的软骨保护作用。本研究通过 GMOCS-Exos 介导的 TGFB1/Nrf2 通路调控为骨关节炎提供了一种有前景的治疗策略。

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